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首页> 美国卫生研究院文献>Clinical Molecular Pathology >Epstein-Barr virus in gastric carcinomas and gastric stump carcinomas: a late event in gastric carcinogenesis
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Epstein-Barr virus in gastric carcinomas and gastric stump carcinomas: a late event in gastric carcinogenesis

机译：胃癌和胃残端癌中的爱泼斯坦-巴尔病毒：胃癌发生的晚期事件

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>Background: To determine at what stage during gastric carcinogenesis Epstein-Barr virus (EBV) enters the gastric epithelial cells, the presence of EBV was investigated in two pathogenetically related but distinct forms of adenocarcinoma of the stomach—gastric carcinoma of the intact stomach (GCIS) and gastric stump carcinoma (GSC)—and their presumed precursor lesions.>Patients and methods: Eleven patients with EBV positive GCIS and eight patients with EBV positive GSC, demonstrated by the highly sensitive EBV encoded RNA 1/2 (EBER1/2) RNA in situ hybridisation (RISH) technique, were studied. Paraffin wax embedded tissue available from preoperative gastric biopsies and tumour adjacent tissue from the resection specimens containing normal gastric mucosa, inflamed gastric mucosa, and preneoplastic lesions (intestinal metaplasia and dysplasia) was investigated by EBER1/2 RISH, in addition to EBV nuclear antigen 1 (EBNA-1) and latent membrane protein 1 (LMP-1) immunohistochemistry (IHC).>Results: In both GCIS and GSC and their precursor lesions EBER1/2 transcripts were restricted to the carcinoma cells. In addition, positivity of EBNA-1 IHC was also restricted to the tumour cells. IHC for LMP-1 was negative in all cases tested.>Conclusions: The absence of EBER1/2 transcripts in preneoplastic gastric lesions (intestinal metaplasia and dysplasia) and their presence in two distinct types of gastric carcinoma strongly suggest that EBV can only infect neoplastic gastric cells and thus is a late event in gastric carcinogenesis.
机译：>背景：为了确定爱泼斯坦-巴尔病毒（EBV）在胃癌发生的哪个阶段进入胃上皮细胞，在两种与胃病相关但不同的腺癌形式中研究了EBV的存在完整的胃癌（GCIS）和胃残端癌（GSC）以及其推测的前体病变。>患者和方法： EBV阳性GCIS的11例患者和EBV阳性GSC的8例，研究了高度敏感的EBV编码的RNA 1/2（EBER1 / 2）RNA原位杂交（RISH）技术。除EBV核抗原1以外，还通过EBER1 / 2 RISH研究了术前胃活检组织中石蜡蜡包埋的组织以及可切除的标本中包含正常胃黏膜，发炎的胃黏膜和肿瘤前病变（肠上皮化生和异型增生）的切除标本的癌旁组织。 （EBNA-1）和潜伏膜蛋白1（LMP-1）免疫组化（IHC）。>结果：在GCIS和GSC及其前体病变中，EBER1 / 2转录本均局限于癌细胞。此外，EBNA-1 IHC的阳性也仅限于肿瘤细胞。在所有测试的病例中，LMP-1的IHC均为阴性。>结论：肿瘤前胃病变（肠化生和异型增生）中不存在EBER1 / 2转录本，并且在两种不同类型的胃癌中均存在EBV只能感染赘生性胃细胞，因此是胃癌发生的晚期事件。

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