首页> 美国卫生研究院文献>Microbial Cell Factories >Directed evolution of single-chain Fv for cytoplasmic expression using the β-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation

【2h】

Directed evolution of single-chain Fv for cytoplasmic expression using the β-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation

机译：使用β-半乳糖苷酶互补检测法指导单链Fv进化为胞质表达可导致高度易受蛋白酶降解和聚集的蛋白质

代理获取

本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。

页面导航

摘要
著录项
相似文献
相关主题

摘要

BackgroundAntibody fragments are molecules widely used for diagnosis and therapy. A large amount of protein is frequently required for such applications. New approaches using folding reporter enzymes have recently been proposed to increase soluble expression of foreign proteins in Escherichia coli. To date, these methods have only been used to screen for proteins with better folding properties but have never been used to select from a large library of mutants. In this paper we apply one of these methods to select mutations that increase the soluble expression of two antibody fragments in the cytoplasm of E. coli.

机译：背景技术抗体片段是广泛用于诊断和治疗的分子。这种应用经常需要大量的蛋白质。最近已经提出了使用折叠报道酶的新方法来增加外源蛋白在大肠杆菌中的可溶性表达。迄今为止，这些方法仅用于筛选具有更好折叠特性的蛋白质，而从未用于从大型突变体文库中进行选择。在本文中，我们应用这些方法之一来选择能增加两个抗体片段在大肠杆菌细胞质中的可溶性表达的突变。

著录项

期刊名称 Microbial Cell Factories
作者
Pascal Philibert; Pierre Martineau;
展开▼
作者单位

展开▼
年(卷),期 2004(3),-1
年度 2004
页码 16
总页数 17
原文格式 PDF
正文语种
中图分类应用微生物学;
关键词

相似文献

外文文献
专利

1. Directed evolution of single-chain Fv for cytoplasmic expression using the β-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation [J] . Pascal Philibert, Pierre Martineau Microbial Cell Factories . 2004,第1期

机译：使用β-半乳糖苷酶互补检测法指导单链Fv进化为胞质表达可导致高度易受蛋白酶降解和聚集的蛋白质
2. Cloning and expression of functional single-chain Fv antibodies directed against NIa and coat proteins of potato virus Y. [J] . Rouis S, Lafaye P, Jaoua Aydi L, Journal of Virological Methods . 2006,第1期

机译：针对马铃薯N病毒的NIa和外壳蛋白的功能性单链Fv抗体的克隆和表达。
3. Cytoplasmic production of soluble and functional single-chain Fv-Fc fusion protein in Escherichia coli [J] . Hiroyuki Sonoda, Yoichi Kumada, Tomohisa Katsuda Biochemical Engineering Journal . 2011,第3期

机译：大肠杆菌中可溶性和功能性单链Fv-Fc融合蛋白的细胞质产生
4. Direct observation of trap behaviors during degradation and breakdown evolution in highly stressed SiO/sub 2/ films by conductive atomic force microscopy [C] . Li Zhang, Milani, Y. Reliability Physics Symposium, 2005. Proceedings. 43rd Annual. 2005 IEEE International . 2005

机译：通过导电原子力显微镜直接观察高应力SiO / sub 2 /薄膜在降解和击穿演化过程中的陷阱行为
5. I. Development of a general method for assaying protein activity in vivo. II. Efforts toward the conversion of a penicillin-binding protein into a beta-lactamase by rational design and directed evolution. [D] . Sengupta, Debleena. 2004

机译：I.开发在体内测定蛋白质活性的通用方法。二。通过合理的设计和定向进化，努力将青霉素结合蛋白转化为β-内酰胺酶。
6. LON is the master protease that protects against protein aggregation in human mitochondria through direct degradation of misfolded proteins [O] . Ayenachew Bezawork-Geleta, Erica J. Brodie, David A. Dougan, -1

机译：LON是主要蛋白酶可通过直接折叠错误折叠的蛋白质来防止人类线粒体中的蛋白质聚集
7. Directed evolution of single-chain Fv for cytoplasmic expression using the β-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation [O] . Martineau Pierre, Philibert Pascal 2004

机译：使用β-半乳糖苷酶互补检测法指导单链Fv进化为胞质表达可导致高度易受蛋白酶降解和聚集的蛋白质
8. Protein Degradation in a TX-TL Cell-free Expression System Using ClpXP Protease. [R] . Sun, Z. Z., Kim, J., Singhal, V., 2014

机译：使用ClpXp蛋白酶在TX-TL无细胞表达系统中降解蛋白质。

Directed evolution of single-chain Fv for cytoplasmic expression using the β-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation

摘要

著录项

相似文献

相关主题

期刊订阅