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In vitro method for directed evolution of proteins, useful e.g. in pharmaceutical development, uses expression system for performing translation, transcription and reverse transcription
In vitro method for directed evolution of proteins, useful e.g. in pharmaceutical development, uses expression system for performing translation, transcription and reverse transcription
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机译：用于蛋白质定向进化的体外方法，例如在药物开发中，使用表达系统进行翻译，转录和逆转录
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摘要
A method for preparing variants (Y') of a protein (Y) that have improved binding properties for a target molecule (X), is new. A method for preparing variants (Y') of a protein (Y), comprises: (a) preparing an in vitro expression system consisting of a nucleic acid sequence (S), encoding Y; a target molecule (X) able to bind to Y and Y'; RNA polymerase (Pol), able to transcribe S; and a reverse transcriptase (RT) able to transcribe the transcript from S, where either X is coupled to Pol and Y to RT; or X is coupled to RT and Y to Pol; (b) incubating the system under conditions that: (a) allow transcription, reverse transcription and translation, to generate Y' (and corresponding coding sequences S'); and (b) favor formation of Y' that have improved binding for X; and (c) isolating Y' with improved binding for X and/or isolation of the corresponding coding sequence S'. An independent claim is also included for a kit for the process.
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