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首页> 美国卫生研究院文献>International Journal of Clinical and Experimental Medicine >Application of loop-mediated isothermal amplification (LAMP) assay for the rapid diagnosis of pathogenic bacteria in clinical sputum specimens of acute exacerbation of COPD (AECOPD)
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Application of loop-mediated isothermal amplification (LAMP) assay for the rapid diagnosis of pathogenic bacteria in clinical sputum specimens of acute exacerbation of COPD (AECOPD)

机译：环介导的等温扩增（LAMP）测定法在快速诊断COPD急性加重的临床痰标本中快速诊断病原菌的应用（AECOPD）

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The present study explores the application of LAMP for rapid diagnosis of pathogenic bacteria in clinical sputum specimens of AECOPD as compared with conventional sputum culturing method. 120 sputum specimens of AECOPD patients, 46 sputum specimens of healthy controls, as well as 166 serum specimens as negative controls, were evaluated by LAMP assay using primers of eight typical respiratory pathogens. No cross-reactivity was observed in these negative control species using LAMP assay. The lower detection limit of LAMP assay was approximately 103 copies. 25 cases (20.8%) were detected at least one positive bacteria species by conventional sputum culturing method, while 73 cases (60.8%) were tested positive in LAMP assay. Moreover, compared with sputum culture, bacterial titers results of LAMP assay were more consistent with FEV1/FVC value of AECOPD patients. These results indicated that the sensitivity of LAMP assay was significantly higher than that of sputum culturing method.
机译：本研究探索了LAMP与常规痰培养方法相比在AECOPD临床痰标本中快速诊断病原菌的应用。使用八种典型呼吸道病原体的引物，通过LAMP分析评估了AECOPD患者的120份痰标本，46份健康对照的痰标本以及166份作为阴性对照的血清标本。使用LAMP分析在这些阴性对照物种中未观察到交叉反应。 LAMP检测的下限约为10 3 。通过常规痰培养方法检测到至少一种阳性细菌的25例（20.8％），而在LAMP分析中检测出73例（60.8％）为阳性。此外，与痰培养相比，LAMP测定的细菌滴度结果与AECOPD患者的FEV1 / FVC值更一致。这些结果表明，LAMP测定的灵敏度显着高于痰培养法。

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