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Resilient microorganisms in dust samples of the International Space Station—survival of the adaptation specialists

机译:国际空间站粉尘样品中的弹性微生物-适应专家的生存

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摘要

BackgroundThe International Space Station (ISS) represents a unique biotope for the human crew but also for introduced microorganisms. Microbes experience selective pressures such as microgravity, desiccation, poor nutrient-availability due to cleaning, and an increased radiation level. We hypothesized that the microbial community inside the ISS is modified by adapting to these stresses.For this reason, we analyzed 8–12 years old dust samples from Russian ISS modules with major focus on the long-time surviving portion of the microbial community. We consequently assessed the cultivable microbiota of these samples in order to analyze their extremotolerant potential against desiccation, heat-shock, and clinically relevant antibiotics. In addition, we studied the bacterial and archaeal communities from the stored Russian dust samples via molecular methods (next-generation sequencing, NGS) and compared our new data with previously derived information from the US American ISS dust microbiome.
机译:背景技术国际空间站(ISS)为机组人员提供了独特的生物群落,也为引入的微生物提供了独特的生物群落。微生物会经受选择性压力,例如微重力,干燥,由于清洁而导致营养物利用率差以及辐射水平增加。我们假设国际空间站内的微生物群落通过适应这些压力而发生了变化。因此,我们分析了来自俄罗斯国际空间站模块的8-12岁的尘埃样品,主要关注微生物群落的长期存活部分。因此,我们评估了这些样品的可培养微生物群,以分析其对脱水,热休克和临床相关抗生素的极端耐受能力。此外,我们通过分子方法(下一代测序,NGS)研究了来自俄罗斯储存的尘埃样品的细菌和古细菌群落,并将我们的新数据与先前从美国ISS尘埃微生物组获得的信息进行了比较。

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