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Phage-associated differences in staphylococcal enterotoxin A gene (sea) expression correlate with sea allele class.

机译:噬菌体肠毒素A基因(海)表达的噬菌体相关差异与海等位基因类别相关。

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摘要

Staphylococcus aureus strains which produced either high or low levels of staphylococcal enterotoxin A (SEA) with a minimal eightfold difference between the two groups were identified. For FRI100 and FRI281A (prototypes for each group), strain differences in the expression of the SEA-encoding gene (sea) were found to occur at the level of sea mRNA concentration, and part of the difference in expression was associated with the sea-containing phages. Southern blot analysis revealed that this phage-associated difference was not due to differences in the copy number of sea. Nucleotide sequence analysis of sea from FRI281A revealed a new allele of sea, with the majority of the sequence differences occurring in the upstream promoter region. Although a strict correlation was observed between the level of SEA production and sea allele class for several strains, the sequence differences observed in the upstream region were not sufficient in themselves to alter the expression level of sea.
机译:鉴定出产生高或低水平的葡萄球菌肠毒素A(SEA)的金黄色葡萄球菌菌株,两组之间的差异最小。对于FRI100和FRI281A(每组的原型),发现SEA编码基因(海)表达的菌株差异发生在海mRNA浓度水平,并且部分表达差异与海-含有噬菌体。 Southern印迹分析表明,这种与噬菌体有关的差异不是由于海拷贝数的差异。来自FRI281A的海核苷酸序列分析揭示了一个新的海等位基因,其中大多数序列差异发生在上游启动子区域。尽管在几种菌株的SEA产生水平和海洋等位基因类别之间观察到严格的相关性,但在上游区域观察到的序列差异本身不足以改变海洋的表达水平。

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