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首页> 美国卫生研究院文献>Infection and Immunity >Comparison among opsonic activity antimeningococcal immunoglobulin G response and serum bactericidal activity against meningococci in sera from vaccinees after immunization with a serogroup B outer membrane vesicle vaccine.
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Comparison among opsonic activity antimeningococcal immunoglobulin G response and serum bactericidal activity against meningococci in sera from vaccinees after immunization with a serogroup B outer membrane vesicle vaccine.

机译：用血清群B外膜囊泡疫苗免疫后被疫苗血清中的调理活性抗脑膜炎球菌免疫球蛋白G反应和血清抗脑膜炎球菌活性的比较。

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Opsonic activity in sera from 27 military recruits vaccinated with the Norwegian meningococcal serogroup B outer membrane vesicle vaccine was measured as respiratory burst with polymorphonuclear leukocytes as the effector cells and meningococci of the epidemic strain as the target. The results were compared with antimeningococcal IgG antibodies against an outer membrane vesicle coat in an enzyme-linked immunosorbent assay and with serum bactericidal activity. The vaccinees were immunized twice, with a 6-week interval between the two. The serum samples studied were collected at day zero, after 6 weeks, and after 12 weeks. Both serum bactericidal activity and respiratory burst were measured by adding external serum as the complement source. The results revealed a significant increase in specific IgG response, serum bactericidal activity, and respiratory burst after vaccination. We found a highly significant correlation between the responses in all three assays (P < 0.0001). The highest correlation was found between respiratory burst and antimeningococcal IgG response (r = 0.93). This result strongly indicates that respiratory burst is mediated almost exclusively by IgG antibodies. The correlation between antimeningococcal IgG response and serum bactericidal activity was slightly lower (r = 0.83). The correlation between respiratory burst and serum bactericidal activity was further reduced (r = 0.78), and some of the sera revealed a marked preference for only one of the activities. This result means that respiratory burst and serum bactericidal activity in part are induced by different mediators, and to obtain a more complete picture of the potential protective activity, both assays should be applied to survey a vaccine trial.
机译：用多形核白细胞作为效应细胞，以流行株的脑膜炎球菌为靶标，对呼吸道爆发进行测量，测量27名接受挪威B型脑膜炎球菌血清群外膜囊泡疫苗接种的新兵血清中的调理活性。在酶联免疫吸附试验中，将结果与针对膜外囊泡涂层的抗脑膜炎球菌IgG抗体以及血清杀菌活性进行了比较。疫苗接种两次，两次间隔6周。在第0天，6周和12周后收集研究的血清样品。通过添加外部血清作为补体来源来测量血清杀菌活性和呼吸爆发。结果表明，接种疫苗后特异性IgG反应，血清杀菌活性和呼吸爆发明显增加。我们发现在所有三种测定中，应答之间具有高度显着的相关性（P <0.0001）。呼吸爆发与抗脑膜炎球菌IgG应答之间的相关性最高（r = 0.93）。该结果强烈表明呼吸爆发几乎完全由IgG抗体介导。抗脑膜炎球菌IgG反应与血清杀菌活性之间的相关性略低（r = 0.83）。呼吸爆发与血清杀菌活性之间的相关性进一步降低（r = 0.78），并且一些血清显示出仅优先选择其中一种活性。该结果意味着呼吸爆发和血清杀菌活性部分是由不同的介质诱导的，并且为了更全面地了解潜在的保护活性，应同时使用两种测定方法来调查疫苗试验。

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