• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Infection and Immunity >Structural Properties of Lipopolysaccharides from Rickettsia typhi and Rickettsia prowazekii and Their Chemical Similarity to the Lipopolysaccharide from Proteus vulgaris OX19 Used in the Weil-Felix Test
【2h】

Structural Properties of Lipopolysaccharides from Rickettsia typhi and Rickettsia prowazekii and Their Chemical Similarity to the Lipopolysaccharide from Proteus vulgaris OX19 Used in the Weil-Felix Test

机译：伤寒立克次体和立氏立克次体的脂多糖的结构特性及其与威尔-费利克斯试验中普通变形杆菌OX19脂多糖的化学相似性

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The lipopolysaccharides (LPSs) isolated from typhus group (TG) rickettsiae Rickettsia typhi and Rickettsia prowazekii were characterized by chemical analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by silver staining. LPSs from two species of TG rickettsiae contained glucose, 3-deoxy-d-manno-octulosonic acid, glucosamine, quinovosamine, phosphate, and fatty acids (β-hydroxylmyristic acid and heneicosanoic acid) but not heptose. The O-polysaccharides of these LPSs were composed of glucose, glucosamine, quinovosamine, and phosphorylated hexosamine. Resolution of these LPSs by their apparent molecular masses by SDS-PAGE showed that they have a common ladder-like pattern. Based on the results of chemical composition and SDS-PAGE pattern, we suggest that these LPSs act as group-specific antigens. Furthermore, glucosamine, quinovosamine, and phosphorylated hexosamine were also found in the O-polysaccharide of the LPS from Proteus vulgaris OX19 used in the Weil-Felix test, suggesting that they may represent the antigens common to LPSs from TG rickettsiae and P. vulgaris OX19.
机译：通过化学分析和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE），然后用银染，对从斑疹伤寒立克次体立克次体立克次体立克次氏立克次体立克次体和立氏立克次体中分离出的脂多糖（LPS）进行表征。来自两种立克次氏体立克次体的LPS含有葡萄糖，3-脱氧-d-甘露糖辛酸，葡糖胺，喹诺酮胺，磷酸盐和脂肪酸（β-羟基亚棕榈酸和庚二酸），但不含庚糖。这些LPS的O-多糖由葡萄糖，氨基葡萄糖，喹诺酮胺和磷酸化的六胺组成。通过SDS-PAGE通过其表观分子质量对这些LPS的拆分显示，它们具有常见的梯形图案。基于化学组成和SDS-PAGE模式的结果，我们建议这些LPS充当组特异性抗原。此外，在Weil-Felix试验中使用的普通变形杆菌OX19的LPS的O多糖中也发现了氨基葡萄糖，喹喔啉胺和磷酸化的六胺，这表明它们可能代表TG立克次氏菌和寻常型假单胞菌OX19的LPS共有的抗原。 。

著录项

相似文献

  • 外文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号