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Analytical study of effective biodegradation of p-cresol using Serratia marcescens ABHI001: application in bioremediation

机译:粘质沙雷氏菌ABHI001有效降解对甲酚的分析研究:在生物修复中的应用

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摘要

This study evaluated the capability of Serratia marcescens ABHI001 to effectively degrade p-cresol through different techniques. The molecular identity of the laboratory isolate S. marcescens ABHI001 was confirmed through the 16S ribosomal DNA gene pattern, and its morphological features were investigated through field-emission scanning electron microscopy. In addition, the degradation behavior of the isolate for cresol was verified using several techniques, including UV–visible spectroscopy, followed by high-performance liquid chromatography (HPLC), gas chromatography, and Fourier transform infrared spectroscopy. The maximum degradation percentage of 85% for p-cresol could be achieved after 18 h of incubation with S. marcescens ABHI001. The formation of p-hydroxybenzaldehyde, p-hydroxybenzoate, and protocatechuate metabolites was confirmed through HPLC. The study results indicate that S. marcescens ABHI001 may have applications in the bioremediation of organic pollutants, such as p-cresol.
机译:这项研究评估了粘质沙雷氏菌ABHI001通过不同技术有效降解对甲酚的能力。通过16S核糖体DNA基因图谱证实了实验室分离的粘菌链霉菌ABHI001的分子身份,并通过场发射扫描电子显微镜研究了其形态特征。此外,使用多种技术验证了分离物对甲酚的降解行为,包括紫外可见光谱法,高效液相色谱法(HPLC),气相色谱法和傅里叶变换红外光谱法。与marcescens S. marcescens ABHI001孵育18小时后,对甲酚的最大降解率可达85%。通过HPLC确认了对羟基苯甲醛,对羟基苯甲酸酯和原儿茶酸酯代谢物的形成。研究结果表明,marcescens ABHI001可能在生物修复有机污染物(例如对甲酚)中有应用。

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