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首页> 美国卫生研究院文献>Materials >Dental Implants with Anti-Biofilm Properties: A Pilot Study for Developing a New Sericin-Based Coating
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Dental Implants with Anti-Biofilm Properties: A Pilot Study for Developing a New Sericin-Based Coating

机译：具有抗生物膜特性的牙科植入物：开发新型以丝胶为基础的涂层的初步研究

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Aim: several strategies have been tested in recent years to prevent bacterial colonization of dental implants. Sericin, one of the two main silk proteins, possesses relevant biological activities and also literature reports about its potential antibacterial properties, but results are discordant and not yet definitive. The aim of this study was to evaluate the effectiveness of different experimental protocols in order to obtain a sericin-based coating on medical grade titanium (Ti) able to reduce microbial adhesion to the dental implant surface. Materials and Methods: different strategies for covalent bonding of sericin to Ti were pursued throughout a multi-step procedure on Ti-6Al-4V disks. The surface of grade 5 Ti was initially immersed in NaOH solution to obtain the exposure of functional -OH groups. Two different silanization strategies were then tested using aminopropyltriethoxysilane (APTES). Eventually, the bonding between silanized Ti-6Al-4V and sericin was obtained with two different crosslinking processes: glutaraldehyde (GLU) or carbodiimide/N-Hydroxy-succinimide (EDC/NHS). Micro-morphological and compositional analyses were performed on the samples at each intermediate step to assess the most effective coating strategy able to optimize the silanization and bioconjugation processes. Microbiological tests on the coated Ti-6Al-4V disks were conducted in vitro using a standard biofilm producer strain of Staphylococcus aureus (ATCC 6538) to quantify the inhibition of microbial biofilm formation (anti-biofilm efficacy) at 24 hours. Results: both silanization techniques resulted in a significant increase of silicon (Si) on the Ti-6Al-4V surfaces etched with NaOH. Differences were found between GLU and EDC/NHS bioconjugation strategies in terms of composition, surface micro-morphology and anti-biofilm efficacy. Ti-6Al-4V samples coated with GLU-bound sericin after silanization obtained via vapor phase deposition proved that this technique is the most convenient and effective coating strategy, resulting in a bacterial inhibition of about 53% in respect to the uncoated Ti-6Al-4V disks. Conclusions: The coating with glutaraldehyde-bound sericin after silanization in the vapor phase showed promising bacterial inhibition values with a significant reduction of S. aureus biofilm. Further studies including higher number of replicates and more peri-implant-relevant microorganisms are needed to evaluate the applicability of this experimental protocol to dental implants.
机译：目的：近年来已经测试了几种策略来防止牙植入物的细菌定植。丝胶蛋白是两种主要的蚕丝蛋白之一，具有相关的生物学活性，也有关于其潜在抗菌特性的文献报道，但结果尚不明确，尚不确定。这项研究的目的是评估不同实验方案的有效性，以便在医学级钛（Ti）上获得能够以丝胶为基础的涂层，从而减少微生物对牙科植入物表面的粘附。材料和方法：在Ti-6Al-4V磁盘的整个多步骤过程中，都采用了将丝胶蛋白与Ti共价键合的不同策略。首先将5 Ti的表面浸入NaOH溶液中，以暴露功能性-OH基团。然后使用氨丙基三乙氧基硅烷（APTES）测试了两种不同的硅烷化策略。最终，硅烷化的Ti-6Al-4V与丝胶蛋白之间的键合通过两种不同的交联工艺获得：戊二醛（GLU）或碳二亚胺/ N-羟基-琥珀酰亚胺（EDC / NHS）。在每个中间步骤对样品进行微观形态和成分分析，以评估能够优化硅烷化和生物结合过程的最有效涂层策略。使用标准的金黄色葡萄球菌生物膜生产菌株（ATCC 6538）在体外对涂有涂层的Ti-6Al-4V圆盘进行了微生物测试，以量化24小时对微生物生物膜形成的抑制作用（抗生物膜功效）。结果：两种硅烷化技术均导致用NaOH蚀刻的Ti-6Al-4V表面上的硅（Si）显着增加。在成分，表面微观形态和抗生物膜功效方面，发现GLU和EDC / NHS生物缀合策略之间存在差异。通过气相沉积获得的硅烷化后涂有GLU结合的丝胶的Ti-6Al-4V样品证明，该技术是最方便，最有效的涂层策略，相对于未涂层的Ti-6Al-，细菌抑制率约为53％。 4V磁盘。结论：汽相硅烷化后，戊二醛结合的丝胶蛋白涂层在气相中显示出令人鼓舞的细菌抑制值，并大大减少了金黄色葡萄球菌的生物膜。需要进一步的研究，包括更多的复制品和更多与种植体周围相关的微生物，以评估该实验方案对牙科种植体的适用性。

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