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首页> 美国卫生研究院文献>Indian Journal of Microbiology >Genetic Variability of Beauveria bassiana and a DNA Marker for Environmental Monitoring of a Highly Virulent Isolate Against Cosmopolites sordidus
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Genetic Variability of Beauveria bassiana and a DNA Marker for Environmental Monitoring of a Highly Virulent Isolate Against Cosmopolites sordidus

机译：球孢白僵菌的遗传变异性和DNA标记用于监测对毒蛇毒的高毒分离株的环境

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摘要

The banana weevil Cosmopolites sordidus (Germar) is one of a number of pests that attack banana crops. The use of the entomopathogenic fungus Beauveria bassiana as a biological control agent for this pest may contribute towards reducing the application of chemical insecticides on banana crops. In this study, the genetic variability of a collection of Brazilian isolates of B. bassiana was evaluated. Samples were obtained from various geographic regions of Brazil, and from different hosts of the Curculionidae family. Based on the DNA fingerprints generated by RAPD and AFLP, we found that 92 and 88 % of the loci were polymorphic, respectively. The B. bassiana isolates were attributed to two genotypic clusters based on the RAPD data, and to three genotypic clusters, when analyzed with AFLP. The nucleotide sequences of nuclear ribosomal DNA intergenic spacers confirmed that all isolates are in fact B. bassiana. Analysis of molecular variance showed that variability among the isolates was not correlated with geographic origin or hosts. A RAPD-specific marker for isolate CG 1024, which is highly virulent to C. sordidus, was cloned and sequenced. Based on the sequences obtained, specific PCR primers BbasCG1024F (5′-TGC GGC TGA GGA GGA CT-3′) and BbasCG1024R (5′-TGC GGC TGA GTG TAG AAC-3′) were designed for detecting and monitoring this isolate in the field.Electronic supplementary materialThe online version of this article (doi:10.1007/s12088-012-0292-9) contains supplementary material, which is available to authorized users.
机译：香蕉象鼻Cosmopolites sordidus（Germar）是多种侵害香蕉作物的害虫之一。使用昆虫病原真菌球孢白僵菌作为该害虫的生物防治剂可能有助于减少化学杀虫剂在香蕉作物上的施用。在这项研究中，评估了巴西芽孢杆菌巴西分离株的遗传变异性。样品是从巴西不同地理区域以及Curculionidae家族的不同寄主中获得的。根据RAPD和AFLP产生的DNA指纹图谱，我们发现92％和88％的基因座分别是多态的。当使用AFLP分析时，根据RAPD数据，球孢杆菌的分离物被归因于两个基因型簇，而归因于三个基因型簇。核糖体DNA基因间隔子的核苷酸序列证实所有分离物实际上都是球孢杆菌。分子变异分析表明，分离株之间的变异性与地理来源或寄主无关。克隆和测序了对毒蛾念珠菌具有高度毒性的分离株CG 1024的RAPD特异性标记。根据获得的序列，设计了特异性PCR引物BbasCG1024F（5'-TGC GGC TGA GGA GGA CT-3'）和BbasCG1024R（5'-TGC GGC TGA GTG TAG AAC-3'）用于检测和监测该分离株。电子补充材料本文的在线版本（doi：10.1007 / s12088-012-0292-9）包含补充材料，可供授权用户使用。

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