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Identification and Characterization of an Invasion Antigen B Gene from the Oral Pathogen Campylobacter rectus

机译:口腔致病性弯曲杆菌弯曲抗原B基因的鉴定与表征

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摘要

The oral bacterium, Campylobacter rectus, is an etiological agent of periodontitis. The virulence genes of C. rectus are largely unknown. The aim of this study was to query C. rectus for the presence of an invasion antigen B (ciaB) gene, which is needed for cell invasion by the related species Campylobacter jejuni. PCR and PCR-walking identified a ciaB from C. rectus. In silico analyses of C. rectus 314 ciaB (Cr-ciaB) revealed an ORF of 1,830 base pairs. The Cr-CiaB protein shared significant sequence identity (BLASTx and phylogeny) with CiaB from related campylobacters. Cr-CiaB is predicted to lack membrane helices, signal peptides, and localizes to the cytoplasm; which are consistent with CiaB proteins. Expression of Cr-ciaB was confirmed with RT-PCR; and potential ciaB genes were detected in eight additional strains of C. rectus. Cr-ciaB is the first CiaB identified from the oral campylobacters.
机译:口腔细菌弯曲杆菌弯曲杆菌是牙周炎的病原体。直肌梭菌的毒力基因在很大程度上是未知的。这项研究的目的是查询直肌梭菌是否存在入侵抗原B(ciaB)基因,而相关物种空肠弯曲菌对细胞进行入侵是必需的。 PCR和PCR步移从直肌梭状芽胞杆菌中鉴定了ciaB。在对直肠直肌314 ciaB(Cr-ciaB)的计算机分析中,发现ORF为1,830个碱基对。 Cr-CiaB蛋白与相关弯曲杆菌的CiaB具有显着的序列同一性(BLASTx和系统发育)。预计Cr-CiaB缺乏膜螺旋,信号肽,并位于细胞质中。与CiaB蛋白一致。用RT-PCR证实了Cr-ciaB的表达。在另外八种直肌梭菌中检测到了潜在的ciaB基因。 Cr- ciaB 是从口腔弯曲杆菌中鉴定出的第一个CiaB。

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