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Expression at a 20L scale and purification of the extracellular domain of the Schistosoma mansoni TSP-2 recombinant protein

机译：曼氏血吸虫TSP-2重组蛋白的20L表达和细胞外结构域的纯化

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摘要

A novel recombinant protein vaccine for human schistosomiasis caused by Schistosoma mansoni is under development. The Sm-TSP-2 schistosomiasis vaccine is comprised of a 9 kDa recombinant protein corresponding to the extracellular domain of a unique S. mansoni tetraspanin. Here, we describe the cloning and the expression of the external loop of Sm-TSP-2 recombinant protein secreted by Pichia Pink™ the process development at 20L scale fermentation, and the two-steps purification, which resulted in a protein recovery yield of 31% and a protein purity of 97%. The developed processes are suitable for the production of purified protein for subsequent formulation and Phase 1 clinical studies.

机译：正在开发一种由曼氏血吸虫引起的用于人类血吸虫病的新型重组蛋白疫苗。 Sm-TSP-2血吸虫病疫苗由9 kDa重组蛋白组成，该蛋白对应于独特的曼氏葡萄球菌四跨膜蛋白的胞外域。在这里，我们描述了毕赤酵母粉红色™分泌的Sm-TSP-2重组蛋白的克隆和表达，以及在20升规模发酵过程中的过程开发以及两步纯化，从而使蛋白质回收率达到了31 ％和97％的蛋白质纯度。所开发的方法适用于纯化蛋白的生产，以用于后续制剂和1期临床研究。

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期刊名称 Human Vaccines Immunotherapeutics
作者
Elena Curti; Clifford Kwityn; Bin Zhan; Portia Gillespie; Jill Brelsford; Vehid Deumic; Jordan Plieskatt; Wanderson C Rezende; Eric Tsao; Bose Kalampanayil; Peter J Hotez; Maria Elena Bottazzi;
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年(卷),期 2013(9),11
年度 2013
页码 2342–2350
总页数 9
原文格式 PDF
正文语种
中图分类免疫学;
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专利

1. Expression at a 20L scale and purification of the extracellular domain of the Schistosoma mansoni TSP-2 recombinant protein: A vaccine candidate for human intestinal schistosomiasis [J] . CurtiE., KwitynC., ZhanB., Human vaccines & immunotherapeutics. . 2013,第11期

机译：曼氏血吸虫TSP-2重组蛋白的20L规模表达和胞外域纯化：人肠道血吸虫病的候选疫苗
2. Biophysical and formulation studies of the Schistosoma mansoni TSP-2 extracellular domain recombinant protein, a lead vaccine candidate antigen for intestinal schistosomiasis [J] . ChengW., CurtiE., RezendeW.C., Human vaccines & immunotherapeutics. . 2013,第11期

机译：曼氏血吸虫TSP-2细胞外域重组蛋白（肠道血吸虫病的主要疫苗候选抗原）的生物物理和制剂研究
3. A large‐scale expression strategy for multimeric extracellular protein complexes using Drosophila S2 cells and its application to the recombinant expression of heterodimeric ligand‐binding domains of taste receptor [J] . Yamashita Atsuko, Nango Eriko, Ashikawa Yuji Protein Science: A Publication of the Protein Society . 2017,第11期

机译：使用果蝇S2细胞的多聚体细胞外蛋白复合物的大规模表达策略及其在味觉受体的杂体二聚体结合结构域的重组表达中的应用
4. Genomics and Gene Expression Management Tools for the Schistosoma Mansoni cDNA Microarray Project [C] . Venancio, T.M., DeMarco, Brazilian Symposium on Bioinformatics(BSB 2005); 20050727-29; Sao Leopoldo(BR) . 2005

机译：曼氏血吸虫cDNA基因芯片计划的基因组学和基因表达管理工具
5. Expression and purification of the extracellular domain of the low-density lipoprotein receptor (LDLR-767) and characterization of two peptides derived from apolipoprotein E helix 4 [D] . Chari, Sunitha 2011

机译：低密度脂蛋白受体（LDLR-767）胞外域的表达和纯化以及载脂蛋白E螺旋4的两种肽的表征
6. Biophysical and formulation studies of the Schistosoma mansoni TSP-2 extracellular domain recombinant protein a lead vaccine candidate antigen for intestinal schistosomiasis [O] . Weiqiang Cheng, Elena Curti, Wanderson C Rezende, 2013

机译：曼氏血吸虫TSP-2细胞外域重组蛋白（肠道血吸虫病的主要疫苗候选抗原）的生物物理和制剂研究
7. A large-scale expression strategy for multimeric extracellular protein complexes using Drosophila S2 cells and its application to the recombinant expression of heterodimeric ligand-binding domains of taste receptor [O] . Atsuko Yamashita, Eriko Nango, Yuji Ashikawa 2017

机译：使用果蝇S2细胞的多聚体细胞外蛋白复合物的大规模表达策略及其在味觉受体的杂体二聚体结合结构域的重组表达中的应用
8. Purification of the Major Concanavalin A-Binding Surface Glycoprotein from Adult Schistosoma mansoni [R] . Hayunga, E. G., Sumner, M. P., Stek, M., 1983

机译：从成年曼氏血吸虫中纯化大型伴刀豆球蛋白a结合表面糖蛋白

Expression at a 20L scale and purification of the extracellular domain of the Schistosoma mansoni TSP-2 recombinant protein

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