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首页> 美国卫生研究院文献>Human Vaccines Immunotherapeutics >Methionine enkephalin (MENK) improves lymphocyte subpopulations in human peripheral blood of 50 cancer patients by inhibiting regulatory T cells (Tregs)
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Methionine enkephalin (MENK) improves lymphocyte subpopulations in human peripheral blood of 50 cancer patients by inhibiting regulatory T cells (Tregs)

机译：甲硫氨酸脑啡肽（MENK）通过抑制调节性T细胞（Tregs）改善50位癌症患者外周血中的淋巴细胞亚群

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摘要

MENK, a penta-peptide is considered as being involved in the regulatory feedback loop between the immune and neuroendocrine systems, with marked modulation of various functions of human immune cells. The aim of the present work was to investigate change of lymphocyte subpopulations in peripheral blood of 50 cancer patients before and after treatment with MENK. Peripheral blood mononuclear cells (PBMCs) of peripheral blood from 50 cancer patients were isolated by density gradient centrifugation using Ficoll-Paque solution and cultured with MENK. We measured proliferation of total nucleated cells, subpopulations of individual CD4+T cells, CD8+T cells, CD4+CD25+ regulatory T cells (Treg), natural killer cells (NK) before and after treatment with 10-12M MENK in cell culture by flow cytometry (FCM). Our results indicated that MENK showed a strong inhibiting effect on Treg cells while it stimulated marked proliferation of other lymphocyte subpopulations. All data obtained were of significance statistically.It was therefore concluded that MENK could work as a strong immune booster with great potential in restoring damaged human immune system and we could consider MENK as a drug to treat cancer patients, whose immune systems are damaged by chemotherapy or radiotherapy. Furthermore we could consider MENK as a chemotherapy additive, which would sustain immune system of cancer patients during the process of chemotherapy to get maximized efficacy with minimized side effect.
机译：五肽MENK被认为参与了免疫系统和神经内分泌系统之间的调节反馈回路，并显着调节了人类免疫细胞的各种功能。本工作的目的是研究MENK治疗前后50名癌症患者外周血淋巴细胞亚群的变化。使用Ficoll-Paque溶液通过密度梯度离心分离来自50位癌症患者的外周血外周血单个核细胞（PBMC），并用MENK培养。我们测量了10 -12 处理前后的总有核细胞增殖，单个CD4 + T细胞亚群，CD8 + T细胞，CD4 + CD25 +调节性T细胞（Treg），自然杀伤细胞（NK）。 sup> M MENK通过流式细胞仪（FCM）进行细胞培养。我们的结果表明，MENK对Treg细胞具有很强的抑制作用，同时它刺激其他淋巴细胞亚群的明显增殖。因此得出的结论是，MENK可以作为强大的免疫促进剂，在恢复受损的人体免疫系统方面具有巨大潜力，我们可以考虑将MENK作为治疗癌症患者的药物，该患者的化疗导致免疫系统受损或放疗。此外，我们可以考虑将MENK用作化学疗法的添加剂，它将在化学疗法过程中维持癌症患者的免疫系统，从而获得最大的功效和最小的副作用。

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