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Genome-wide DNA binding pattern of the homeodomain transcription factor Sine oculis (So) in the developing eye of Drosophila melanogaster

机译:果蝇的发育眼中同源域转录因子Sine oculis(So)的全基因组DNA结合模式

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摘要

The eye of the fruit fly Drosophila melanogaster provides a highly tractable genetic model system for the study of animal development, and many genes that regulate Drosophila eye formation have homologs implicated in human development and disease. Among these is the homeobox gene sine oculis (so), which encodes a homeodomain transcription factor (TF) that is both necessary for eye development and sufficient to reprogram a subset of cells outside the normal eye field toward an eye fate. We have performed a genome-wide analysis of So binding to DNA prepared from developing Drosophila eye tissue in order to identify candidate direct targets of So-mediated transcriptional regulation, as described in our recent article . The data are available from NCBI Gene Expression Omnibus (GEO) with the accession number . Here we describe the methods, data analysis, and quality control of our So ChIP-seq dataset.
机译:果蝇的果蝇的果蝇的眼睛为研究动物的发育提供了一个易于处理的遗传模型系统,许多调节果蝇的眼睛形成的基因都与人类的发育和疾病有关。其中有一个同源异型盒基因正弦(soine)基因,它编码同源异域转录因子(TF),这对于眼睛发育是必需的,并且足以将正常眼野外的一部分细胞重新编程为命运。如我们最近的文章中所述,我们已经对So结合至从发育中的果蝇眼组织制备的DNA进行了全基因组分析,以鉴定So介导的转录调控的候选直接靶标。数据可从NCBI基因表达综合总线(GEO)获得,登录号为。在这里,我们描述了So ChIP-seq数据集的方法,数据分析和质量控制。

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