• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Genes >Listeria monocytogenes Isolated from Illegally Imported Food Products into the European Union Harbor Different Virulence Factor Variants
【2h】

Listeria monocytogenes Isolated from Illegally Imported Food Products into the European Union Harbor Different Virulence Factor Variants

机译：从非法进口食品中分离出单核细胞增生李斯特菌进入欧盟港口的各种毒力因子变异体

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Unregulated international flow of foods poses a danger to human health, as it may be contaminated with pathogens. Recent studies have investigated neglected routes of pathogen transmission and reported the occurrence of Listeria monocytogenes in food illegally imported into the European Union (EU), either confiscated at four international airports or sold illegally on the Romanian black market. In this study we investigated the genotype diversity and the amino acid sequence variability of three main virulence factors of 57 L. monocytogenes isolates. These isolates were derived from 1474 food samples illegally imported into the EU and originated from 17 different countries. Multilocus sequence typing revealed 16 different sequence types (STs) indicating moderate genotype diversity. The most prevalent STs were ST2, ST9, and ST121. The pulsed-field gel electrophoresis (PFGE) analysis resulted in 34 unique pulsotypes. PFGE types assigned to the most prevalent STs (ST2, ST9, and ST121) were highly related in their genetic fingerprint. Internalin A (InlA) was present in 20 variants, including six truncated InlA variants, all harbored by isolates of ST9 and ST121. We detected eight ST-specific listeriolysin O (LLO) variants, and among them, one truncated form. The actin-assembly-inducing protein ActA was present in 15 different ST-specific variants, including four ActA variants with an internal truncation. In conclusion, this study shows that L. monocytogenes, isolated from illegally imported food, have moderate genotype diversity, but diverse virulence factors variants, mainly of InlA.
机译：不受管制的国际食品流通对人类健康构成威胁，因为它可能被病原体污染。最近的研究已经调查了被忽略的病原体传播途径，并报告了在四个国际机场被没收或在罗马尼亚黑市上非法出售的，非法进口到欧盟（EU）食品中单核细胞增生李斯特菌的发生。在这项研究中，我们调查了57种单核细胞增生李斯特菌菌株的三个主要毒力因子的基因型多样性和氨基酸序列变异性。这些分离株来自非法进口到欧盟的1474种食品样品，来自17个不同国家。多基因座序列分型揭示了16种不同的序列类型（ST），表明中等的基因型多样性。最流行的ST是ST2，ST9和ST121。脉冲场凝胶电泳（PFGE）分析产生了34种独特的脉冲型。分配给最流行的ST（ST2，ST9和ST121）的PFGE类型在其遗传指纹中高度相关。 Internalin A（InlA）存在于20个变体中，包括6个截短的InlA变体，所有变体都由ST9和ST121的分离物所掩藏。我们检测到八种ST特异性李斯特菌溶血素O（LLO）变体，其中一种是截短形式。肌动蛋白装配诱导蛋白ActA存在于15种不同的ST特异变体中，包括四个具有内部截短的ActA变体。总之，这项研究表明，从非法进口的食物中分离出的单核细胞增生李斯特菌具有中等的基因型多样性，但毒力因子变异多样，主要是InlA。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号