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首页> 美国卫生研究院文献>G3: GenesGenomesGenetics >Identification of Genes Uniquely Expressed in the Germ-Line Tissues of the Jewel Wasp Nasonia vitripennis
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Identification of Genes Uniquely Expressed in the Germ-Line Tissues of the Jewel Wasp Nasonia vitripennis

机译：鉴定珠宝黄蜂Nasonia vitripennis胚芽组织中独特表达的基因

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The jewel wasp Nasonia vitripennis is a rising model organism for the study of haplo-diploid reproduction characteristic of hymenopteran insects, which include all wasps, bees, and ants. We performed transcriptional profiling of the ovary, the female soma, and the male soma of N. vitripennis to complement a previously existing transcriptome of the wasp testis. These data were deposited into an open-access genome browser for visualization of transcripts relative to their gene models. We used these data to identify the assemblies of genes uniquely expressed in the germ-line tissues. We found that 156 protein-coding genes are expressed exclusively in the wasp testis compared with only 22 in the ovary. Of the testis-specific genes, eight are candidates for male-specific DNA packaging proteins known as protamines. We found very similar expression patterns of centrosome associated genes in the testis and ovary, arguing that de novo centrosome formation, a key process for development of unfertilized eggs into males, likely does not rely on large-scale transcriptional differences between these tissues. In contrast, a number of meiosis-related genes show a bias toward testis-specific expression, despite the lack of true meiosis in N. vitripennis males. These patterns may reflect an unexpected complexity of male gamete production in the haploid males of this organism. Broadly, these data add to the growing number of genomic and genetic tools available in N. vitripennis for addressing important biological questions in this rising insect model organism.
机译：宝石黄蜂Nasonia vitripennis是一种新兴的模式生物，用于研究膜翅目昆虫的单倍体-二倍体繁殖特征，其中包括所有黄蜂，蜜蜂和蚂蚁。我们对N. vitripennis的卵巢，雌性体细胞和雄性体进行转录谱分析，以补充黄蜂睾丸的先前转录组。这些数据被存入开放访问的基因组浏览器中，用于相对于其基因模型的转录物的可视化。我们使用这些数据来确定在种系组织中独特表达的基因的组装。我们发现，在黄蜂睾丸中仅表达156个蛋白质编码基因，而在卵巢中仅表达22个。在睾丸特异性基因中，有八种是男性特异性DNA包装蛋白（称为鱼精蛋白）的候选物。我们在睾丸和卵巢中发现了与中心体相关基因非常相似的表达模式，认为从头中心体形成是未受精卵发育成雄性的关键过程，很可能不依赖于这些组织之间的大规模转录差异。相比之下，尽管减数分枝杆菌男性中没有真正的减数分裂，但许多与减数分裂相关的基因仍偏向于睾丸特异性表达。这些模式可能反映了这种生物的单倍体雄性配子生产中出乎意料的复杂性。广泛地讲，这些数据增加了玻璃trip猪笼草（N. vitripennis）中可用于解决这种新兴昆虫模型生物中重要生物学问题的越来越多的基因组和遗传工具。

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