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>Protective effects of hydrogen rich water on the intestinal ischemia/reperfusion injury due to intestinal intussusception in a rat model
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Protective effects of hydrogen rich water on the intestinal ischemia/reperfusion injury due to intestinal intussusception in a rat model
This study aimed to investigate the protective effects of hydrogen rich water on the intestinal ischemia/reperfusion (I/R) injury in a rat intestinal intussusception (II) model. Ninety Sprague-Dawley rats were randomly assigned into three groups (n = 30 per group). In sham group, rats received laparotomy, and the intestine was exposed for 15 minutes without II. In I/R + saline group and I/R + hydrogen group, rats received II after laparotomy and then intestine was relocated 8 hours later, followed by immediately intraperitoneal injection of normal saline and hydrogen rich water (HRW) (5 mL/kg), respectively. One hour later, the intestine was collected for hematoxylin-eosin staining and immunohistochemistry for apoptotic cells and 8-oxo-deoxyguanosine, and blood was harvested for detection of tumor necrosis factor-α, malondialdehyde and superoxide dismutase. Hematoxylin-eosin staining showed the intestinal mucosa was significantly damaged in I/R + saline group, which was markedly attenuated after HRW treatment. The serum tumor necrosis factor-α content increased significantly in I/R + saline group, but HRW treatment reduced serum tumor necrosis factor-α content as compared to I/R + saline group (P < 0.05). Serum malondialdehyde content and 8-oxo-deoxyguanosine positive cells in the intestine increased dramatically after II, but HRW significantly reduced them in I/R+hydrogen group (P < 0.05). In addition, superoxide dismutase activity reduced markedly and apoptotic cells increased in I/R + saline group as compared to sham group, but they HRW increased superoxide dismutase activity and reduced apoptotic cells significantly in I/R + hydrogen group (P < 0.05). Our results indicate hydrogen rich water is able to attenuate II induced intestinal I/R injury via inhibiting intestinal inflammation, attenuating intestinal/serum oxidative stress and reducing apoptotic intestinal cells.
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机译：这项研究旨在调查富氢水对大鼠肠套叠（II）模型肠缺血/再灌注（I / R）损伤的保护作用。将90只Sprague-Dawley大鼠随机分为三组（每组30只）。在假手术组中，大鼠接受剖腹手术，并且在没有II的情况下将肠暴露15分钟。在I / R +盐水组和I / R +氢组中，大鼠在剖腹手术后接受II，然后在8小时后将肠重新定位，然后立即腹膜内注射生理盐水和富氢水（HRW）（5 mL / kg） ， 分别。一小时后，收集肠进行苏木精-伊红染色，并对凋亡细胞和8-氧代-脱氧鸟苷进行免疫组织化学，并收集血液以检测肿瘤坏死因子-α，丙二醛和超氧化物歧化酶。苏木精-伊红染色显示，I / R +盐水组肠粘膜明显受损，HRW处理后肠黏膜明显减弱。 I / R +生理盐水组血清肿瘤坏死因子-α含量显着增加，但HRW治疗较I / R +生理盐水组降低血清肿瘤坏死因子-α含量（P <0.05）。 II期后，肠中的血清丙二醛含量和8-氧代-脱氧鸟苷阳性细胞显着增加，但HRW在I / R +氢组中显着降低它们（P <0.05）。此外，与假手术组相比，I / R +盐水组的超氧化物歧化酶活性显着降低，凋亡细胞增加，但HRW在I / R +氢组的超氧化物歧化酶活性显着降低，凋亡细胞显着减少（P <0.05）。我们的结果表明，富氢水能够通过抑制肠道炎症，减轻肠道/血清氧化应激和减少凋亡的肠道细胞来减轻II型肠道I / R损伤。
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