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首页> 外文期刊>Digestive Diseases and Sciences >Protective Effects of Leflunomide on Intestinal Ischemia-Reperfusion Injury Leflunomide against intestinal ischemia-reperfusion
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Protective Effects of Leflunomide on Intestinal Ischemia-Reperfusion Injury Leflunomide against intestinal ischemia-reperfusion

机译：来氟米特对肠缺血再灌注损伤的保护作用来氟米特对肠缺血再灌注损伤的保护作用

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Aim The aim of this study was to investigate the possible protective effects of leflunomide, which has antioxidant and anti-inflammatory properties, against intestinal IR injury in rats. Materials and Methods Forty female Wistar albino rats were divided into six groups: control (n = 5), drug control (n = 7), sham operated (n = 7), IR alone (n = 7), IR plus vehicle (IR + vehicle, n = 7) and IR plus 20 mg/kg leflunomide (IR + Leflunomide, n = 7). While rats were pretreated intragastrically with leflunomide (20 mg/kg) and vehicle in three doses prior to the experiment, respectively, in the IR + Leflunomide and IR + vehicle groups, no additional application was done in the IR alone group. Intestines were exteriorized, and the superior mesenteric artery was occluded for 45 min ischemia, and then the clamp was removed for 120 min reperfusion. After the experiment, the intestines were removed for biochemical and histological examinations. Additionally, blood samples were taken for measurements of antioxidant parameters. Results The intestinal IR significantly increased the MDA level and MPO activity; however, treatment with leflunomide reversed those findings (P < 0.05). The CAT activity of the IR + Leflunomide group was significantly higher than in the IR groups (P < 0.05). The SOD activity was increased in the intestinal IR group, and leflunomide treatment reversed that, too (P <0.05). The light microscopic findings showed that IR caused mucosal necrosis and leflunomide treatment reduced the morphological alterations associated with IR (P < 0.05). Conclusion Intestinal IR injury may be reversed by the anti-inflammatory and antioxidant actions of leflunomide. Keywords Intestinal ischemia-reperfusion - Leflunomide - Oxidant - Myeloperoxidase - Antioxidants - Antiinflammatory
机译：目的本研究的目的是研究具有抗氧化和抗炎特性的来氟米特对大鼠肠道IR损伤的可能保护作用。材料和方法40只雌性Wistar白化病大鼠分为六组：对照组（n = 5），药物对照组（n = 7），假手术（n = 7），仅IR（n = 7），IR加赋形剂（IR） +媒介物，n = 7）和IR加20 mg / kg来氟米特（IR +来氟米特，n = 7）。在实验前，分别在IR +来氟米特和IR +载体组中分别以三剂量的来氟米特（20 mg / kg）和溶媒对大鼠进行了胃内预处理，而在单独IR组中未进行其他应用。将肠外化，并阻塞肠系膜上动脉进行45分钟的缺血，然后取下固定夹进行120分钟的再灌注。实验后，将肠移出进行生化和组织学检查。另外，采集血样以测量抗氧化剂参数。结果肠道IR显着增加MDA水平和MPO活性。但是，来氟米特治疗逆转了这些发现（P <0.05）。 IR +来氟米特组的CAT活性显着高于IR组（P <0.05）。肠道IR组SOD活性增加，来氟米特治疗也将其逆转（P <0.05）。光学显微镜结果表明，IR引起粘膜坏死，来氟米特治疗减少了与IR相关的形态学改变（P <0.05）。结论来氟米特的抗炎和抗氧化作用可逆转肠道IR损伤。关键词肠缺血再灌注-来氟米特-氧化剂-髓过氧化物酶-抗氧化剂-抗炎

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