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首页> 美国卫生研究院文献>Frontiers in Pharmacology >A Combined LC-MS Metabolomics- and 16S rRNA Sequencing Platform to Assess Interactions between Herbal Medicinal Products and Human Gut Bacteria in Vitro: a Pilot Study on Willow Bark Extract
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A Combined LC-MS Metabolomics- and 16S rRNA Sequencing Platform to Assess Interactions between Herbal Medicinal Products and Human Gut Bacteria in Vitro: a Pilot Study on Willow Bark Extract

机译：组合的LC-MS代谢组学和16S rRNA测序平台以评估草药和人类肠道细菌之间的相互作用：柳树皮提取物的初步研究

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Herbal preparations are complex mixtures of natural products, many of which are able to reach the distal gut due to low oral bioavailability. There, they can influence the microbial communities, and can be metabolized into potentially absorbable bioactive compounds by the intestinal bacteria. This aspect has often been disregarded when searching for the active principles of medicinal plants and herbal medicinal products. The aim of this study was to establish an interdisciplinary platform to unravel interactions of herbal medicine and intestinal microbiota, using a combined LC-MS metabolomics and 16S rRNA microbiome sequencing approach. Willow bark extract (WBE), a herbal medicinal product with a long history of traditional use and a well-established anti-inflammatory activity, was incubated with human fecal suspension under anoxic conditions. Samples were taken after 0.5, 4, and 24 h of incubation. Microbiome analyses revealed that incubation with WBE had a marked effect on microbial community composition and functions. For example, the proportion of Bacteroides sp. was clearly enhanced when the fecal sample used in this study was incubated with WBE. LC-MS analysis showed that WBE constituents were readily metabolized by fecal bacteria. Numerous microbial metabolites could be annotated, allowing the construction of putative microbial degradation pathways for the main groups of WBE constituents. We suggest that studies of this type help to increase the knowledge on bioactive principles of medicinal plants, since gut microbial metabolites might have been underestimated as a source of bioactive compounds in the past.
机译：草药制剂是天然产品的复杂混合物，由于口服生物利用度低，许多产品都可以到达远端肠道。在那里，它们可以影响微生物群落，并可以被肠道细菌代谢为潜在可吸收的生物活性化合物。在寻找药用植物和草药产品的有效成分时，经常会忽略这一方面。这项研究的目的是建立一个跨学科的平台，以结合使用LC-MS代谢组学和16S rRNA微生物组测序方法来揭示草药与肠道菌群的相互作用。将柳树皮提取物（WBE），一种具有悠久传统使用历史并具有公认的抗炎活性的草药，在缺氧条件下与人粪便悬浮液一起孵育。孵育0.5、4和24小时后取样。微生物组分析显示，与WBE一起孵育对微生物群落组成和功能有显着影响。例如，拟杆菌的比例。当将本研究中使用的粪便样品与WBE一起孵育时，其明显增强。 LC-MS分析表明，WBE成分容易被粪便细菌代谢。可以注释许多微生物代谢产物，从而为WBE主要成分组建立假定的微生物降解途径。我们建议这种类型的研究有助于增加对药用植物生物活性原理的认识，因为过去肠道微生物代谢物可能被低估为生物活性化合物的来源。

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