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首页> 美国卫生研究院文献>Frontiers in Aging Neuroscience >Validation of Minimally-Invasive Sample Collection Methods for Measurement of Telomere Length
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Validation of Minimally-Invasive Sample Collection Methods for Measurement of Telomere Length

机译：微创样本采集方法用于端粒长度测量的验证

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>Objective: The discovery of telomere length (TL) as a biomarker of cellular aging and correlate of age-related disease has generated a new field of research in the biology of healthy aging. Although the most common method of sample collection for TL is venous blood draw, less-invasive DNA collection methods are becoming more widely used. However, how TL relates across tissues derived from these sample collection methods is poorly understood. The current study is the first to characterize the associations in TL across three sample collection methods: venous whole blood, finger prick dried blood spot and saliva.>Methods: TL was measured in 24 healthy young adults using three modes of sample collection for each participant: venous whole blood, finger prick dried blood spot and saliva. Relative TL was measured using quantitative polymerase chain reaction.>Results: TL in finger prick dried blood spots (DBS) washighly correlated with TL in whole blood (r = 0.84, p < 0.001). Salivary TL was also correlated with whole blood TL (r = 0.56, p = 0.005), but this association was not as strong as that of dried blood spot TL (Steiger’s Z = 2.12, p = 0.034). TL was longer in saliva than in whole blood or DBS (p’s < 0.001).>Conclusions: These findings have important implications for future study design by supporting the validity of less-invasive methods that can be implemented with vulnerable populations or in the field. Further, these findings aid in interpreting the burgeoning area of biological aging research and may shed light on our understanding of inconsistencies in the empirical literature.
机译：>目的：端粒长度（TL）作为细胞衰老的生物标志物以及与年龄相关疾病的相关因素的发现，为健康衰老的生物学研究开辟了新领域。尽管最常见的TL样本采集方法是抽血，但侵入性较小的DNA采集方法正变得越来越广泛。然而，人们对TL如何在源自这些样品收集方法的组织中产生关联的了解很少。本研究是第一个通过三种样本采集方法表征TL关联的方法：静脉全血，手指点刺的干血斑和唾液。>方法：使用三种模式对24位健康的年轻人进行了TL测定每个参与者的样本采集：静脉全血，手指刺干的血斑和唾液。使用定量聚合酶链反应测量相对TL。>结果：手指刺干血斑（DBS）中的TL与全血中TL高度相关（r = 0.84，p <0.001）。唾液TL也与全血TL相关（r = 0.56，p = 0.005），但这种关联性不如干血斑TL（Steiger's Z = 2.12，p = 0.034）强。唾液中的TL比全血或DBS的更长（p <0.001）。>结论：这些发现通过支持可在弱势人群中实施的微创方法的有效性，对未来的研究设计具有重要意义。人口或现场。此外，这些发现有助于解释生物老化研究的新兴领域，并可能有助于我们理解经验文献中的不一致之处。

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