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Microbial Biogeography of Arctic Streams: Exploring Influences of Lithology and Habitat

机译:北极溪流的微生物生物地理学:探索岩性和栖息地的影响

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摘要

Terminal restriction fragment length polymorphism and 16S rRNA gene sequencing were used to explore the community composition of bacterial communities in biofilms on sediments (epipssamon) and rocks (epilithon) in stream reaches that drain watersheds with contrasting lithologies in the Noatak National Preserve, Alaska. Bacterial community composition varied primarily by stream habitat and secondarily by lithology. Positive correlations were detected between bacterial community structure and nutrients, base cations, and dissolved organic carbon. Our results showed significant differences at the stream habitat, between epipssamon and epilithon bacterial communities, which we expected. Our results also showed significant differences at the landscape scale that could be related to different lithologies and associated stream biogeochemistry. These results provide insight into the bacterial community composition of little known and pristine arctic stream ecosystems and illustrate how differences in the lithology, soils, and vegetation community of the terrestrial environment interact to influence stream bacterial taxonomic richness and composition.
机译:使用末端限制性片段长度多态性和16S rRNA基因测序来研究阿拉斯加Noatak国家级自然保护区的沉积物(表观)和岩石(表石)上流域的生物膜中细菌群落的群落组成,这些流域具有截然相反的岩性。细菌群落组成主要因溪流生境而异,其次因岩性而异。细菌群落结构与养分,碱性阳离子和溶解的有机碳之间存在正相关。我们的研究结果表明,我们期望的Epipssamon和Epilithon细菌群落之间在河流栖息地存在显着差异。我们的结果还显示出景观尺度上的显着差异,可能与不同的岩性和相关的河流生物地球化学有关。这些结果提供了对鲜为人知的原始北极流生态系统细菌群落组成的见解,并说明了陆地环境的岩性,土壤和植被群落的差异如何相互作用以影响河流细菌分类学的丰富性和组成。

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