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首页> 美国卫生研究院文献>Frontiers in Microbiology >Stratified active archaeal communities in the sediments of Jiulong River estuary China
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Stratified active archaeal communities in the sediments of Jiulong River estuary China

机译：九龙江河口沉积物中的层状活跃古细菌群落

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Here the composition of total and active archaeal communities in a sediment core of Jiulong River estuary at Fujian Province, Southern China was reported. Profiles of CH4 and SO2−4 concentrations from the sediment core indicated the existence of a sulfate-methane transition zone (SMTZ) in which sulfate reduction-coupled anaerobic oxidation of methane (AOM) occurs. Accordingly, three sediment layers (16–18.5 cm, 71–73.5 cm, and 161–163.5 cm) from the 1.2 m sediment core were sectioned and named top, middle and bottom, respectively. Total DNA and RNA of each layer were extracted and used for clone libraries and sequence analysis of 16S rRNA genes, the reverse transcription (RT)-PCR products of 16S rRNA and methyl CoM reductase alpha subunit (mcrA) genes. Phylogenetic analysis indicated that archaeal communities of the three layers were dominated by the Miscellaneous Crenarchaeotal Group (MCG) whose ecological functions were still unknown. The MCG could be further divided into seven subgroups, named MCG-A, B, C, D, E, F, and G. MCG-A and MCG-G were the most active groups in the estuarine sediments. Known anaerobic methanotrophic archaea (ANMEs) were only found as minor components in these estuarine archaeal communities. This study, together with the studies of deep subsurface sediments, would be a very good start point to target and compare the specific active archaeal groups and their roles in the dark, deep subsurface sediment environments.
机译：在此，报道了中国南方福建省九龙河口沉积物核心中的总古细菌群落和活跃古细菌群落的组成。沉积物中心的CH4和SO <2-4>浓度曲线表明存在硫酸盐-甲烷过渡带（SMTZ），在其中发生了硫酸盐还原耦合的厌氧甲烷氧化（AOM）。因此，从1.2 m的沉积岩心中分离出三个沉积层（16–18.5 cm，71–73.5 cm和161–163.5 cm），并分别命名为顶部，中部和底部。提取每层的总DNA和RNA，用于克隆文库和16S rRNA基因的序列分析，16S rRNA和甲基CoM还原酶α亚基（mcrA）基因的逆转录（RT）-PCR产物。系统发育分析表明，这三层的古细菌群落主要由杂种的nar鱼科（MCG）主导，其生态功能尚不清楚。 MCG可以进一步分为七个亚组，分别为MCG-A，B，C，D，E，F和G。MCG-A和MCG-G是河口沉积物中最活跃的组。已知的厌氧甲烷营养古细菌（ANME）仅在这些河口古细菌群落中被发现为次要成分。这项研究以及对深层地下沉积物的研究，将是针对和比较特定的活性古细菌群体及其在黑暗，深层地下沉积物环境中的作用的一个很好的起点。

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