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首页> 美国卫生研究院文献>Frontiers in Endocrinology >Different distribution of neuromedin S and its mRNA in the rat brain: NMS peptide is present not only in the hypothalamus as the mRNA but also in the brainstem
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Different distribution of neuromedin S and its mRNA in the rat brain: NMS peptide is present not only in the hypothalamus as the mRNA but also in the brainstem

机译：神经调节素S及其mRNA在大鼠脑中的分布不同：NMS肽不仅以下丘脑的形式存在于mRNA而且存在于脑干中。

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摘要

Neuromedin S (NMS) is a neuropeptide identified as another endogenous ligand for two orphan G protein-coupled receptors, FM-3/GPR66 and FM-4/TGR-1, which have also been identified as types 1 and 2 receptors for neuromedin U structurally related to NMS. Although expression of NMS mRNA is found mainly in the brain, spleen, and testis, the distribution of its peptide has not yet been investigated. Using a newly prepared antiserum, we developed a highly sensitive radioimmunoassay for rat NMS. NMS peptide was clearly detected in the rat brain at a concentration of 68.3 ± 3.4 fmol/g wet weight, but it was hardly detected in the spleen and testis. A high content of NMS peptide was found in the hypothalamus, midbrain, and pons–medulla oblongata, whereas abundant expression of NMS mRNA was detected only in the hypothalamus. These differing distributions of the mRNA and peptide suggest that nerve fibers originating from hypothalamic NMS neurons project into the midbrain, pons, or medulla oblongata. In addition, abundant expression of type 2 receptor mRNA was detected not only in the hypothalamus, but also in the midbrain and pons–medulla oblongata. These results suggest novel, unknown physiological roles of NMS within the brainstem.
机译：Neuromedin S（NMS）是一种神经肽，被鉴定为两个孤儿G蛋白偶联受体FM-3 / GPR66和FM-4 / TGR-1的另一种内源性配体，它们也已被确定为Neuromedin U的1型和2型受体在结构上与NMS有关。尽管NMS mRNA的表达主要存在于大脑，脾脏和睾丸中，但尚未研究其肽的分布。使用新制备的抗血清，我们为大鼠NMS开发了一种高度灵敏的放射免疫分析方法。 NMS肽在大鼠脑中的湿重为68.3±3.4 fmol / g的浓度清晰可见，但在脾脏和睾丸中几乎未检出。在下丘脑，中脑和脑桥延髓中发现高含量的NMS肽，而仅在下丘脑中检测到NMS mRNA的大量表达。 mRNA和肽的这些不同分布表明，源自下丘脑NMS神经元的神经纤维伸入中脑，脑桥或延髓。此外，不仅在下丘脑，而且在中脑和脑桥延髓中都检测到了2型受体mRNA的大量表达。这些结果表明脑干内NMS的新颖，未知的生理作用。

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