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首页> 美国卫生研究院文献>Frontiers in Cellular and Infection Microbiology >Complete Genome Characterization of the 2017 Dengue Outbreak in Xishuangbanna a Border City of China Burma and Laos
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Complete Genome Characterization of the 2017 Dengue Outbreak in Xishuangbanna a Border City of China Burma and Laos

机译：中国缅甸和老挝边境城市西双版纳2017年登革热暴发的完整基因组特征

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A dengue outbreak abruptly occurred at the border of China, Myanmar, and Laos in June 2017. By November 3rd 2017, 1184 infected individuals were confirmed as NS1-positivein Xishuangbanna, a city located at the border. To verify the causative agent, complete genome information was obtained through PCR and sequencing based on the viral RNAs extracted from patient samples. Phylogenetic trees were constructed by the maximum likelihood method (MEGA 6.0). Nucleotide and amino acid substitutions were analyzed by BioEdit, followed by RNA secondary structure prediction of untranslated regions (UTRs) and protein secondary structure prediction in coding sequences (CDSs). Strains YN2, YN17741, and YN176272 were isolated from local residents. Stains MY21 and MY22 were isolated from Burmese travelers. The complete genome sequences of the five isolates were 10,735 nucleotides in length. Phylogenetic analysis classified all five isolates as genotype I of DENV-1, while isolates of local residents and Burmese travelers belonged to different branches. The three locally isolates were most similar to the Dongguan strain in 2011, and the other two isolates from Burmese travelers were most similar to the Laos strain in 2008. Twenty-four amino acid substitutions were important in eight evolutionary tree branches. Comparison with DENV-1SS revealed 658 base substitutions in the local isolates, except for two mutations exclusive to YN17741, resulting in 87 synonymous mutations. Compared with the local isolates, 52 amino acid mutations occurred in the CDS of two isolates from Burmese travelers. Comparing MY21 with MY22, 17 amino acid mutations were observed, all these mutations occurred in the CDS of non-structured proteins (two in NS1, 10 in NS2, two in NS3, three in NS5). Secondary structure prediction revealed 46 changes in the potential nucleotide and protein binding sites of the CDSs in local isolates. RNA secondary structure prediction also showed base changes in the 3′UTR of local isolates, leading to two significant changes in the RNA secondary structure. To our knowledge, this study is the first complete genome analysis of isolates from the 2017 dengue outbreak that occurred at the border areas of China, Burma, and Laos.
机译：2017年6月，在中国，缅甸和老挝边境突然爆发了登革热。到2017年11月3日，在边境的西双版纳市已确诊1184名NS1阳性感染者。为了验证病原体，基于从患者样品中提取的病毒RNA，通过PCR和测序获得了完整的基因组信息。系统进化树通过最大似然法（MEGA 6.0）构建。通过BioEdit分析核苷酸和氨基酸取代，然后进行非翻译区域（UTRs）的RNA二级结构预测和编码序列（CDSs）中的蛋白质二级结构预测。菌株YN2，YN17741和YN176272从当地居民中分离出来。从缅甸旅客身上分离出MY21和MY22污渍。这五个分离株的完整基因组序列长度为10,735个核苷酸。系统发育分析将所有五个分离株归为DENV-1的基因型I，而当地居民和缅甸旅行者的分离株属于不同的分支。 2011年，三个本​​地分离株与东莞菌株最相似，2008年，来自缅甸旅行者的其他两个分离株与老挝菌株最相似。在八个进化树分支中，二十四个氨基酸取代非常重要。与DENV-1SS的比较显示，除了YN17741独有的两个突变外，本地分离株中有658个碱基取代，导致87个同义突变。与本地分离物相比，来自缅甸旅行者的两个分离物的CDS中发生了52个氨基酸突变。比较MY21和MY22，观察到17个氨基酸突变，所有这些突变都发生在非结构蛋白的CDS中（NS1中两个，NS2中10个，NS3中两个，NS5中三个）。二级结构预测揭示了本地分离物中CDS潜在核苷酸和蛋白质结合位点的46个变化。 RNA二级结构预测还显示了局部分离株3'UTR的碱基变化，导致RNA二级结构发生了两个显着变化。据我们所知，这项研究是对2017年在中国，缅甸和老挝边境地区发生的登革热疫情的分离株的首次完整基因组分析。

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