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Carotenoids from Foods of Plant Animal and Marine Origin: An Efficient HPLC-DAD Separation Method

机译：植物动物和海洋食品中的类胡萝卜素：高效液相色谱-DAD分离方法

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Carotenoids are important antioxidant compounds, present in many foods of plant, animal and marine origin. The aim of the present study was to describe the carotenoid composition of tomato waste, prawn muscle and cephalothorax and avian (duck and goose) egg yolks through the use of a modified gradient elution HPLC method with a C30 reversed-phase column for the efficient separation and analysis of carotenoids and their cis-isomers. Elution time was reduced from 60 to 45 min without affecting the separation efficiency. All-trans lycopene predominated in tomato waste, followed by all-trans-β-carotene, 13-cis-lutein and all-trans lutein, while minor amounts of 9-cis-lutein, 13-cis-β-carotene and 9-cis-β-carotene were also detected. Considering the above findings, tomato waste is confirmed to be an excellent source of recovering carotenoids, especially all-trans lycopene, for commercial use. Xanthophylls were the major carotenoids of avian egg yolks, all-trans lutein and all-trans zeaxanthin in duck and goose egg yolk, respectively. In the Penaeus kerathurus prawn, several carotenoids (zeaxanthin, all-trans-lutein, canthaxanthin, cryptoxanthin, optical and geometrical astaxanthin isomers) were identified in considerable amounts by the same method. A major advantage of this HPLC method was the efficient separation of carotenoids and their cis-isomers, originating from a wide range of matrices.
机译：类胡萝卜素是重要的抗氧化剂化合物，存在于许多植物，动物和海洋食品中。本研究的目的是通过使用改进的梯度洗脱HPLC方法和C30反相色谱柱来描述番茄废料，对虾肌肉和头胸部以及禽类（鸭和鹅）蛋黄的类胡萝卜素成分，以进行有效分离和类胡萝卜素及其顺式异构体的分析。洗脱时间从60分钟减少到45分钟，而不会影响分离效率。全反式番茄红素在番茄废料中占主导地位，其次是全反式-β-胡萝卜素，13-顺式叶黄素和全反式叶黄素，而少量的9-顺式-叶黄素，13-顺式-β-胡萝卜素和9-还检测到顺式-β-胡萝卜素。考虑到以上发现，西红柿废料被证实是回收类胡萝卜素，特别是全反式番茄红素用于商业用途的极好来源。叶黄素是鸭蛋黄和鹅蛋黄中禽蛋黄，全反叶黄素和全反玉米黄质的主要类胡萝卜素。在对虾对虾中，通过相同的方法可以鉴定出大量的类胡萝卜素（玉米黄质，全反叶黄素，角黄素，隐黄质，光学和几何虾青素异构体）。 HPLC方法的主要优点是有效分离类胡萝卜素及其顺式异构体，该类胡萝卜素源自多种基质。

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