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首页> 美国卫生研究院文献>Food Science of Animal Resources >Development of Rapid Diagnostic Kit for Identification of Hanwoo (Korean Native Cattle) Brand Meat by Detecting BIO-TAG
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Development of Rapid Diagnostic Kit for Identification of Hanwoo (Korean Native Cattle) Brand Meat by Detecting BIO-TAG

机译：通过检测BIO-TAG鉴定韩宇（韩土）肉品牌快速诊断试剂盒的开发

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This study was performed to develop a rapid immuno-assay kit, by using a specific antigen to detect Hanwoo brand meat. We selected a synthetic antigen specific to our target antibody, named BIO-TAG (Tyr-D-Ala-Phe), by utilizing a computer-based analysis and literature review. BIO-TAG tagged with adjuvant was subcutaneously injected in sheep and Hanwoo. The serum and meat juice of the immunized or non-immunized animal were then analyzed, to measure the titer of antibody by ELISA and Western blot. The amount of antibodies against the BIO-TAG increased (p<0.05) in serum by vaccination. Furthermore, meat juice from the immunized Hanwoo showed greater (p<0.05) antibody titer, compared with those from non-immunized groups. To optimze the dilution factor, we performed dot-ELISA, with various combination levels of BIO-TAG. Results from dot-ELISA showed that 2 mg/mL BIO-TAG was sufficient to distinguish the immunized meat from non-immunized groups. These results support our hypothesis that simple immunization of Hanwoo generates a sufficient amount of antibodies to be detectable in the meat juice by means of the immune-assay. Therefore, specific Hanwoo brand meat can be more precisely identified by our rapid diagnostic kit. This technology can deter possible fraud of counterfeit meat brands in the Korean domestic market with ease and rapidity; and offers a new tool that guarantees consumers high quality Hanwoo brand beef.
机译：进行这项研究以开发一种快速的免疫测定试剂盒，方法是使用特异性抗原检测Hanwoo品牌的肉。通过利用基于计算机的分析和文献综述，我们选择了一种特异性针对我们目标抗体的合成抗原，称为BIO-TAG（Tyr-D-Ala-Phe）。将带有佐剂标签的BIO-TAG皮下注射到绵羊和Hanwoo中。然后分析经免疫或未经免疫的动物的血清和肉汁，以通过ELISA和蛋白质印迹法测量抗体的效价。接种疫苗后血清中针对BIO-TAG的抗体量增加（p <0.05）。此外，与未免疫组相比，已免疫Hanwoo的肉汁显示出更高的（p <0.05）抗体效价。为了优化稀释因子，我们进行了BIO-TAG各种组合水平的点ELISA。点酶联免疫吸附试验的结果表明，2 mg / mL的BIO-TAG足以将免疫肉与未免疫组区分开。这些结果支持了我们的假设，即通过免疫测定，对Hanwoo进行简单的免疫可产生足够量的抗体，以便在肉汁中检测到。因此，通过我们的快速诊断工具可以更精确地识别特定的Hanwoo品牌肉。该技术可以轻松，迅速地阻止韩国国内市场上假冒肉类品牌的欺诈行为；并提供了一种新工具，可确保消费者获得优质的Hanwoo品牌牛肉。

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