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首页> 美国卫生研究院文献>Food Science and Biotechnology >Development and validation of a modified QuEChERS method coupled with LC-MS/MS to determine arbutin in pear peels
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Development and validation of a modified QuEChERS method coupled with LC-MS/MS to determine arbutin in pear peels

机译：改进的QuEChERS方法与LC-MS / MS结合用于测定梨皮中熊果苷的开发和验证

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摘要

A new effective method was developed to determine the concentration of arbutin in pear peels using a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The original QuEChERS was modified to enable the extraction of the polar arbutin molecule. Use of an initial 50:50 acetonitrile:water extraction solvent led to the highest extraction efficiency. The arbutin extracted from pear peels was found to be identical to the β-arbutin standard, as confirmed by NMR and LC-MS/MS analyses. For quantitative analysis, the mass spectra of the precursor ion [M+NH4]+ at m/z 290.0 and the product ion of arbutin at m/z 163.0 were used. The limit of detection, limit of quantification, linearity, precision, accuracy, and recovery of the proposed method were evaluated. We successfully applied this method to pear samples and it may be suitable for the quantitative analysis of arbutin in other similar plant materials.
机译：开发了一种新的有效方法，使用改良的QuEChERS（快速，简便，廉价，有效，坚固和安全）方法和液相色谱-串联质谱（LC-MS / MS）测定梨果皮中熊果苷的浓度。对原始QuEChERS进行了修饰，以能够提取极性熊果苷分子。最初使用50:50的乙腈：水萃取溶剂可获得最高的萃取效率。经NMR和LC-MS / MS分析证实，从梨皮中提取的熊果苷与β-熊果苷标准品相同。为了进行定量分析，使用了m / z 290.0的前体离子[M + NH4] + 的质谱和m / z 163.0的熊果苷的产物离子。评价了该方法的检出限，定量限，线性，精密度，准确度和回收率。我们成功地将该方法应用于梨样品，它可能适用于其他相似植物材料中熊果苷的定量分析。

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