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首页> 美国卫生研究院文献>The EMBO Journal >IS10/Tn10 transposition efficiently accommodates diverse transposon end configurations.
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IS10/Tn10 transposition efficiently accommodates diverse transposon end configurations.

机译：IS10 / Tn10换位可有效适应多种转座子末端构型。

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摘要

Transposon Tn10 and its component insertion sequence IS10 move by non-replicative transposition. We have studied the array of reaction intermediates and products in a high efficiency in vitro IS10/Tn10 transposition reaction. Synapsis of two transposon ends, followed by cleavage and strand transfer, can occur very efficiently irrespective of the relative locations and orientations of the two ends. The two participating ends can occur in inverted or direct orientation on the same molecule or, most importantly, on two different molecules. This behavior contrasts sharply with that of Mu, in which transposition is strongly biased in favor of inverted repeat synapsis. Mechanistically, the absence of discrimination amongst various end configurations implies that the architecture within the IS10/Tn10 synaptic complex is relatively simple, i.e. lacking any significant intertwining of component DNA strands. Biologically these observations are important because they suggest that the IS10 insertion sequence module has considerable flexibility in the types of DNA rearrangements that it can promote. Most importantly, it now seems highly probable that a single non-replicative IS10 element can promote DNA rearrangements usually attributed to replicative transposition, i.e. adjacent deletions and cointegrates, by utilizing transposon ends on two sister chromosomes. Other events which probably also contribute to the diversity of IS10/Tn10-promoted rearrangements are discussed.
机译：转座子Tn10及其成分插入序列IS10通过非复制性转座而移动。我们已经研究了高效的体外IS10 / Tn10转座反应中反应中间体和产物的排列。不论两个末端的相对位置和方向如何，都可以非常有效地发生两个转座子末端的突触，然后发生切割和链转移。两个参与末端可以以颠倒或直接取向出现在同一分子上，或者最重要的是，出现在两个不同的分子上。这种行为与Mu的行为形成鲜明对比，后者的移位强烈偏向于反向重复突触。从机械上讲，在各种末端构型之间没有区别意味着在IS10 / Tn10突触复合物中的结构是相对简单的，即没有任何明显的组分DNA链缠结。从生物学上讲，这些观察很重要，因为它们表明IS10插入序列模块在其可促进的DNA重排类型中具有相当大的灵活性。最重要的是，现在似乎很可能单个非复制IS10元件可以利用两个姐妹染色体上的转座子末端来促进通常归因于复制转座的DNA重排，即相邻的缺失和共整合。讨论了可能也有助于IS10 / Tn10促进的重排多样性的其他事件。

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