首页> 美国卫生研究院文献>Mass Spectrometry >Separation of Isobaric Compounds Using a Spiral Orbit Type Time-of-Flight Mass Spectrometer MALDI-SpiralTOF
【2h】

Separation of Isobaric Compounds Using a Spiral Orbit Type Time-of-Flight Mass Spectrometer MALDI-SpiralTOF

机译:使用螺旋轨道型飞行时间质谱仪MALDI-SpiralTOF分离同量化合物

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

The development of a MALDI-TOF mass spectrometer that utilizes spiral ion trajectory, SpiralTOF, is reported. The total flight path length was 17 m, which is five times longer than that in commonly used reflectron ion optical system. The SpiralTOF reduced the dependence of the mass resolving power on the mass of the analyte, while improving the accuracy of the mass measurements. Furthermore, SpiralTOF has two advantages that can be exploited for the separation of minor abundant isobaric components in mass spectra. One is the reduction in chemical background due to the post source decay (PSD), which is achieved through PSD ion elimination by electrostatic sectors contained within the SpiralTOF. The other is that the stabilities of peak positions are improved during mass spectrum accumulation. The peak drift caused by the fine structure of matrix crystals and the small irregularities on the sample surface can be reduced by extending the flight path. In this study, these advantages are demonstrated via the analysis of a block copolymer and mass spectrometry imaging (MSI) of lipids.
机译:据报道,利用螺旋离子轨迹的MALDI-TOF质谱仪SpiralTOF的开发。总飞行路径长度为17μm,是常用反射电子离子光学系统的五倍。 SpiralTOF降低了质量分辨能力对分析物质量的依赖性,同时提高了质量测量的准确性。此外,SpiralTOF具有两个优点,可用于分离质谱图中的次要丰富的等压组分。一种是由于源后衰减(PSD)导致的化学本底降低,这是通过SpiralTOF中包含的静电扇区消除PSD离子来实现的。另一个是在质谱积累过程中提高了峰位置的稳定性。通过延长飞行路径,可以减少由基体晶体的精细结构和样品表面微小不规则引起的峰漂移。在这项研究中,通过对嵌段共聚物的分析和脂质的质谱成像(MSI)证明了这些优势。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号