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Cytochrome P450 102A2 Catalyzes Efficient Oxidation of Sodium Dodecyl Sulphate: A Molecular Tool for Remediation

机译：细胞色素P450 102A2催化十二烷基硫酸钠的有效氧化：修复的分子工具。

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Bacterial cytochrome P450s (CYPs) constitute an important family of monooxygenase enzymes that carry out essential roles in the metabolism of endogenous compounds and foreign chemicals. In the present work we report the characterization of CYP102A2 from B. subtilis with a focus on its substrate specificity. CYP102A2 is more active in oxidation of sodium dodecyl sulphate (SDS) than any other characterized CYP. The effect of SDS and NADPH concentration on reaction rate showed nonhyperbolic and hyperbolic dependence, respectively. The enzyme was found to exhibit a bell-shaped curve for plots of activity versus pH, over pH values 5.9–8.5. The rate of SDS oxidation reached the maximum value approximately at pH 7.2 and the pH transition observed controlled by two pK as in the acidic (pK a = 6.7 ± 0.08) and basic (pK a = 7.3 ± 0.06) pH range. The results are discussed in relation to the future biotechnology applications of CYPs.

机译：细菌细胞色素P450（CYP）构成了重要的单加氧酶酶家族，这些酶在内源性化合物和外来化学物质的代谢中起重要作用。在本工作中，我们报道了来自枯草芽孢杆菌的CYP102A2的表征，重点是其底物特异性。 CYP102A2在十二烷基硫酸钠（SDS）的氧化中比任何其他特征性CYP活性更高。 SDS和NADPH浓度对反应速率的影响分别显示为非双曲线和双曲线依赖性。发现该酶在5.9-8.5的pH值下，活性与pH的关系曲线呈钟形曲线。 SDS氧化速率大约在pH 7.2时达到最大值，并且观察到的pH过渡受两个pK控制，如在酸性（pK a = 6.7±0.08）和碱性（pK a = 7.3±0.06）pH范围内。讨论了有关CYP未来生物技术应用的结果。

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期刊名称 Enzyme Research
作者
Irene Axarli; Ariadne Prigipaki; Nikolaos E. Labrou;
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年(卷),期 2010(2010),-1
年度 2010
页码 125429
总页数 7
原文格式 PDF
正文语种
中图分类分子生物学;
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专利

1. Cytochrome P450 102A2 Catalyzes Efficient Oxidation of Sodium Dodecyl Sulphate: A Molecular Tool for Remediation [J] . IreneAxarli, AriadnePrigipaki, Nikolaos E.Labrou Enzyme Research . 2010,第2期

机译：细胞色素P450 102A2催化十二烷基硫酸钠的有效氧化：修复的分子工具。
2. The cytochrome c-catalysed oxidation of ascorbate in the presence of sodium dodecyl sulphate and NNN'N'-tetramethyl-p-phenylenediamine [J] . J M Hill The biochemical journal . 1970,第4期

机译：在十二烷基硫酸钠和NNN'N'-四甲基-对苯二胺的存在下，细胞色素c催化抗坏血酸的氧化
3. Roles of NADPH-P450 reductase and apo- and holo-cytochrome b(5) on xenobiotic oxidations catalyzed by 12 recombinant human cytochrome P450s expressed in membranes of Escherichia coli [J] . Yamazaki H., Nakamura M., Komatsu T., Protein Expression and Purification . 2002,第3期

机译：NADPH-P450还原酶和脱辅基和全细胞色素b（5）在大肠杆菌膜上表达的12种重组人细胞色素P450催化的异种生物氧化中的作用
4. Development of the Stereoselective Oxidation System Catalyzed by Cytochrome P450BM3 Using Decoy Molecules [C] . Kazuto Suzuki, Osami Shoji, Joshua Kyle Stanfield 日本化学会春季年会講演予稿集 . 2018

机译：诱饵分子催化细胞色素P450BM3催化的立体选择性氧化体系
5. Model Complexes for Reactive Intermediates in Cytochrome P450 Nitric Oxide Reductase (P450nor). [D] . McQuarters, Ashley B. 2017

机译：细胞色素P450一氧化氮还原酶（P450nor）中反应性中间体的模型复合物。
6. The cytochrome c-catalysed oxidation of ascorbate in the presence of sodium dodecyl sulphate and NNNN-tetramethyl-p-phenylenediamine. [O] . J M Hill 1970

机译：在十二烷基硫酸钠和NNNN-四甲基-对苯二胺的存在下细胞色素c催化抗坏血酸的氧化。
7. Cytochrome P450 102A2 Catalyzes Efficient Oxidation of Sodium Dodecyl Sulphate: A Molecular Tool for Remediation [O] . Axarli, Irene, Prigipaki, Ariadne, Labrou, Nikolaos E. 2010

机译：细胞色素P450 102A2催化十二烷基硫酸钠的有效氧化：修复的分子工具。

Cytochrome P450 102A2 Catalyzes Efficient Oxidation of Sodium Dodecyl Sulphate: A Molecular Tool for Remediation

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