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首页> 美国卫生研究院文献>The EMBO Journal >Structures and physiological roles of 13 integral lipids of bovine heart cytochrome c oxidase
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Structures and physiological roles of 13 integral lipids of bovine heart cytochrome c oxidase

机译：牛心脏细胞色素c氧化酶13种整体脂质的结构和生理作用

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All 13 lipids, including two cardiolipins, one phosphatidylcholine, three phosphatidylethanolamines, four phosphatidylglycerols and three triglycerides, were identified in a crystalline bovine heart cytochrome c oxidase (CcO) preparation. The chain lengths and unsaturated bond positions of the fatty acid moieties determined by mass spectrometry suggest that each lipid head group identifies its specific binding site within CcOs. The X-ray structure demonstrates that the flexibility of the fatty acid tails facilitates their effective space-filling functions and that the four phospholipids stabilize the CcO dimer. Binding of dicyclohexylcarbodiimide to the O2 transfer pathway of CcO causes two palmitate tails of phosphatidylglycerols to block the pathway, suggesting that the palmitates control the O2 transfer process.The phosphatidylglycerol with vaccenate (cis-Δ11-octadecenoate) was found in CcOs of bovine and Paracoccus denitrificans, the ancestor of mitochondrion, indicating that the vaccenate is conserved in bovine CcO in spite of the abundance of oleate (cis-Δ9-octadecenoate). The X-ray structure indicates that the protein moiety selects cis-vaccenate near the O2 transfer pathway against trans-vaccenate. These results suggest that vaccenate plays a critical role in the O2 transfer mechanism.
机译：在结晶牛心脏细胞色素C氧化酶（CcO）制剂中鉴定出所有13种脂质，包括两种心磷脂，一种磷脂酰胆碱，三种磷脂酰乙醇胺，四种磷脂酰甘油和三种甘油三酸酯。通过质谱法测定的脂肪酸部分的链长和不饱和键位置表明，每个脂质头基团都可识别其在CcOs中的特异性结合位点。 X射线结构表明，脂肪酸尾部的柔韧性有助于其有效的空间填充功能，并且四个磷脂可稳定CcO二聚体。二环己基碳二亚胺与CcO的O2转移途径结合导致磷脂酰甘油的两个棕榈酸酯尾部阻断该途径，表明该棕榈酸酯控制着O2转移过程。磷脂酰甘油与疏松剂（cis-Δ 11 -octadecenoate）在线粒体的祖先牛和反硝化球菌的CcO中发现了CcO，这表明尽管油酸含量很高（顺式Δ 9 -octadecenoate），但在牛CcO中仍保留了真空。 X射线结构表明该蛋白部分选择了O 2转移途径附近的顺式-反义酸来抵抗反式-反义酸。这些结果表明，接种疫苗在氧气转移机制中起着至关重要的作用。

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