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首页> 外文期刊>Angewandte Chemie >X-ray Crystal Structures of Cytochrome c Oxidases from Paracoccus denitrificans and Bovine Heart and Their Implications for the Molecular Mechanism of Cell Respiration
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X-ray Crystal Structures of Cytochrome c Oxidases from Paracoccus denitrificans and Bovine Heart and Their Implications for the Molecular Mechanism of Cell Respiration

机译：反硝化球菌和牛心脏细胞色素c氧化酶的X射线晶体结构及其对细胞呼吸分子机制的影响

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Thirty-four years ago. two years after the first high-resolution X-ray structure analysis of a protein (myoglobin) by Kendrew, crystals of cytochrome c oxidase (COX) from bovine heart were obtained by Yonetani. But all efforts to determine the three-dimensional structure of the enzyme at the atomic level failed due to the poor quality of crystals. Now high-resolution (2.8 A) X-ray crystal structures of the oxidized COX from Para-coccus denitrificans and from bovine heart have been published almost simultaneously by Michel and co-workers. and by Yoshikawa and co-workers. respectively. In the two cases, however, different approaches were used. Michel et al. obtained crystals of COX from Paracoccus only after forming a complex with the variable fragment of a monoclonal antibody, whereas Yoshikawa et al. obtained crystals of COX from bovine heart only after addition of the detergent decylmaltoside during crystallization.
机译：三十四年前。在Kendrew对蛋白质（肌红蛋白）进行首次高分辨率X射线结构分析之后的两年，Yonenetani获得了来自牛心脏的细胞色素c氧化酶（COX）晶体。但是，由于晶体质量差，所有在原子级确定酶三维结构的努力都失败了。现在，米歇尔及其同事几乎同时发表了脱硝副球菌和牛心脏中氧化的COX的高分辨率（2.8 A）X射线晶体结构。以及吉川及其同事。分别。但是，在两种情况下，使用了不同的方法。 Michel等。仅在与单克隆抗体的可变片段形成复合物后，才从副球菌中获得了COX的晶体，而Yoshikawa等人。仅在结晶过程中添加去污剂癸基麦芽糖苷后，才能从牛心获得COX晶体。

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