class='kwd-title'>Method name: Soil DNA/RNA co-e'/> Modification of a commercial DNA extraction kit for safe and rapid recovery of DNA and RNA simultaneously from soil without the use of harmful solvents
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>MethodsX >Modification of a commercial DNA extraction kit for safe and rapid recovery of DNA and RNA simultaneously from soil without the use of harmful solvents
【2h】

Modification of a commercial DNA extraction kit for safe and rapid recovery of DNA and RNA simultaneously from soil without the use of harmful solvents

机译：改进了商用DNA提取试剂盒可同时从土壤中安全快速地回收DNA和RNA而无需使用有害溶剂

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

class="kwd-title">Method name: Soil DNA/RNA co-extraction class="kwd-title">Keywords: Soil DNA and RNA, Quantitative co-extraction, Molecular biomass, Tropical soil class="head no_bottom_margin" id="idm139719489392704title">AbstractAn optimized method, based on the coupling of two commercial kits, is described for the extraction of soil nucleic acids, with simultaneous extraction and purification of DNA and RNA following a cascade scheme and avoiding the use of harmful solvents. The protocol canmonitor the variations in the recovery yield of DNA and RNA from soils of various types.The quantitative version of the protocol was obtained by testing the starting soil quantity, the grinding parameters and the final elution volumes, in order to avoid saturation of both kits. class="first-line-outdent">
  • • A first soil-crushing step in liquid nitrogen could be added for the assessment of fungal parameters.
  • • The protocol was efficienton different tropical soils, including Andosol, while their high contents of clays, including poorly crystalline clays, and Fe and Al oxides usually make the nucleic acid extraction more difficult.
  • • The RNA recovery yield from the previous tropical soils appeared to correlate better to soil respiration than DNA, which is positively influenced by soil clay content.
    • 机译：<！-fig ft0-> <！-fig @ position =“ anchor” mode =文章f4-> <！-fig mode =“ anchred” f5-> <！-fig / graphic | fig / alternatives / graphic mode =“ anchored” m1-> class =“ kwd-title”>方法名称：土壤DNA / RNA共提取 class =“ kwd-title”>关键字：< / strong>土壤DNA和RNA，定量共提取，分子生物量，热带土壤 class =“ head no_bottom_margin” id =“ idm139719489392704title”>摘要一种基于两种商业试剂盒的优化方法，描述了用于提取土壤核酸的方法，同时遵循级联方案并避免使用有害溶剂，同时提取和纯化DNA和RNA。该方案可以监测各种类型土壤中DNA和RNA回收率的变化。该方案的定量版本是通过测试土壤的起始量，研磨参数和最终洗脱量而获得的，以避免两者均饱和 class =“ first-line-outdent”> <！-list-behavior =简单的前缀-word = mark-type = none max-label-size = 9->
  • •可以添加液氮中的第一步土壤破碎步骤来评估真菌参数。
  • •该协议对包括Andosol在内的各种热带土壤有效，而粘土含量高，包括结晶性较差的粘土，以及铁和铝的氧化物通常使核酸提取更加困难。
  • •从先前的热带土壤中回收的RNA似乎比土壤呼吸具有更好的相关性DNA，它受到土壤黏土含量的积极影响。

    • 著录项

    相似文献

    • 外文文献
    • 中文文献
    • 专利
    代理获取

    客服邮箱：kefu@zhangqiaokeyan.com

    京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

    • 客服微信

    • 服务号