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首页> 美国卫生研究院文献>Microbes and Environments >Uranium (U)-Tolerant Bacterial Diversity from U Ore Deposit of Domiasiat in North-East India and Its Prospective Utilisation in Bioremediation
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Uranium (U)-Tolerant Bacterial Diversity from U Ore Deposit of Domiasiat in North-East India and Its Prospective Utilisation in Bioremediation

机译：印度东北多米亚西亚铀矿床中的铀耐受细菌多样性及其在生物修复中的前瞻性利用

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Uranium (U)-tolerant aerobic chemo-heterotrophic bacteria were isolated from the sub-surface soils of U-rich deposits in Domiasiat, North East India. The bacterial community explored at molecular level by amplified ribosomal DNA restriction analysis (ARDRA) resulted in 51 distinct phylotypes. Bacterial community assemblages at the U mining site with the concentration of U ranging from 20 to 100 ppm, were found to be most diverse. Representative bacteria analysed by 16S rRNA gene sequencing were affiliated to Firmicutes (51%), Gammaproteobacteria (26%), Actinobacteria (11%), Bacteroidetes (10%) and Betaproteobacteria (2%). Representative strains removed more than 90% and 53% of U from 100 μM and 2 mM uranyl nitrate solutions, respectively, at pH 3.5 within 10 min of exposure and the activity was retained until 24 h. Overall, 76% of characterized isolates possessed phosphatase enzyme and 53% had PIB-type ATPase genes. This study generated baseline information on the diverse indigenous U-tolerant bacteria which could serve as an indicator to estimate the environmental impact expected to be caused by mining in the future. Also, these natural isolates efficient in uranium binding and harbouring phosphatase enzyme and metal-transporting genes could possibly play a vital role in the bioremediation of metal-/radionuclide-contaminated environments.
机译：从印度东北多米亚西亚特富铀矿床的地下土壤中分离出了耐铀（U）的好氧化学异养细菌。通过扩增的核糖体DNA限制性分析（ARDRA）在分子水平上探索的细菌群落产生了51种不同的系统型。在U矿区，U浓度为20至100 ppm的细菌群落集合最为多样。通过16S rRNA基因测序分析的代表性细菌属于Firmicutes（51％），γ-变形杆菌（26％），Actinobacteria（11％），Bacteroidetes（10％）和Betaproteobacteria（2％）。代表性菌株在暴露后10分钟内，在pH 3.5时，分别从100μM和2 mM硝酸铀酰溶液中去除了90％以上和53％的U，活性保持到24小时。总体而言，76％的特征分离株具有磷酸酶，53％具有PIB型ATPase基因。这项研究生成了有关多种本地耐U细菌的基线信息，这些基线信息可以用作估算未来采矿对环境造成的影响的指标。同样，这些有效分离铀并保留磷酸酶和金属转运基因的天然分离株可能在金属/放射性核素污染环境的生物修复中发挥重要作用。

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