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首页> 美国卫生研究院文献>The Korean Journal of Physiology Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology >Relaxant Effect of Spermidine on Acethylcholine and High K+-induced Gastric Contractions of Guinea-Pig
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Relaxant Effect of Spermidine on Acethylcholine and High K+-induced Gastric Contractions of Guinea-Pig

机译：亚精胺对乙酰胆碱和高K +诱导的豚鼠胃收缩的放松作用

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In our previous study, we found that spermine and putrescine inhibited spontaneous and acetylcholine (ACh)-induced contractions of guinea-pig stomach via inhibition of L-type voltage-dependent calcium current (VDCCL). In this study, we also studied the effect of spermidine on mechanical contractions and calcium channel current (IBa), and then compared its effects to those by spermine and putrescine. Spermidine inhibited spontaneous contraction of the gastric smooth muscle in a concentration-dependent manner (IC50=1.1±0.11 mM). Relationship between inhibition of contraction and calcium current by spermidine was studied using 50 mM high K+-induced contraction: Spermidine (5 mM) significantly reduced high K+ (50 mM)-induced contraction to 37±4.7% of the control (p<0.05), and inhibitory effect of spermidine on IBa was also observed at a wide range of test potential in current/voltage (I/V) relationship. Pre- and post-application of spermidine (5 mM) also significantly inhibited carbachol (CCh) and ACh-induced initial and phasic contractions. Finally, caffeine (10 mM)-induced contraction which is activated by Ca2+-induced Ca2+ release (CICR),' was also inhibited by pretreatment of spermidine (5 mM). These findings suggest that spermidine inhibits spontaneous and CCh-induced contraction via inhibition of VDCCL and Ca2+ releasing mechanism in guinea-pig stomach.
机译：在我们以前的研究中，我们发现精胺和腐胺通过抑制L型电压依赖性钙电流（VDCCL）抑制豚鼠胃的自发性和乙酰胆碱（ACh）诱导的收缩。在这项研究中，我们还研究了亚精胺对机械收缩和钙通道电流（IBa）的作用，然后将其与精胺和腐胺的作用进行了比较。亚精胺以浓度依赖的方式抑制胃平滑肌的自发收缩（IC50 = 1.1±0.11 mM）。用50 mM高K + 诱导的收缩研究了亚精胺抑制收缩与钙电流之间的关系：亚精胺（5 mM）显着降低了高K + （50 mM）诱导的收缩至对照的37±4.7％（p <0.05），并且在电流/电压（I / V）关系的广泛测试电位下，也观察到亚精胺对IBa的抑制作用。亚精胺（5 mM）的前后施用也显着抑制了卡巴胆碱（CCh）和ACh诱导的初始和阶段性收缩。最后，咖啡因（10 mM）引起的收缩被Ca 2 + 诱导的Ca 2 + 释放（CICR）激活，也被亚精胺预处理抑制了（ 5毫米）。这些发现提示亚精胺通过抑制豚鼠胃中的VDCCL和Ca 2 + 释放机制来抑制自发性和CCh诱导的收缩。

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