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Gene-sized macronuclear DNA molecules are clustered in micronuclear chromosomes of the ciliate Oxytricha nova.

机译:基因大小的大核DNA分子聚集在纤毛虫Oxytricha nova的微核染色体中。

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摘要

Following the sexual phase of its life cycle, the hypotrichous ciliate Oxytricha nova transforms a copy of its chromosomal micronucleus into a macronucleus containing short, linear DNA molecules with an average size of 2.2 kilobase pairs. In addition, more than 90% of the DNA sequences in the micronuclear genome are eliminated during this process. We have examined the organization of macronuclear DNA molecules in the micronuclear chromosomes. Macronuclear DNA molecules were found to be clustered and separated by less than 550 base pairs in two cloned segments of micronuclear DNA. Recombinant clones of two macronuclear DNA molecules that are adjacent in the micronucleus were also isolated and examined by DNA sequencing. The two macronuclear DNA molecules were found to be separated by only 90 base pairs in the micronuclear genome.
机译:在其生命周期的性阶段之后,毛纤毛虫Oxytricha nova将其染色体微核的一个副本转换为一个包含短而线性的DNA分子的大核,其平均大小为2.2 kb。此外,在此过程中,微核基因组中超过90%的DNA序列被清除。我们已经检查了微核染色体中大核DNA分子的组织。发现大核DNA分子在两个克隆的微核DNA片段中聚集并被少于550个碱基对隔开。还分离了在微核中相邻的两个大核DNA分子的重组克隆,并通过DNA测序进行了检查。发现两个大核DNA分子在微核基因组中仅被90个碱基对隔开。

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