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首页> 外文期刊>Molecular and Cellular Biology >Gene-sized macronuclear DNA molecules are clustered in micronuclear chromosomes of the ciliate Oxytricha nova.
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Gene-sized macronuclear DNA molecules are clustered in micronuclear chromosomes of the ciliate Oxytricha nova.

机译：基因大小的常规DNA分子聚集在CiLiatiate onytricha Nova的微核染色体中。

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Following the sexual phase of its life cycle, the hypotrichous ciliate Oxytricha nova transforms a copy of its chromosomal micronucleus into a macronucleus containing short, linear DNA molecules with an average size of 2.2 kilobase pairs. In addition, more than 90% of the DNA sequences in the micronuclear genome are eliminated during this process. We have examined the organization of macronuclear DNA molecules in the micronuclear chromosomes. Macronuclear DNA molecules were found to be clustered and separated by less than 550 base pairs in two cloned segments of micronuclear DNA. Recombinant clones of two macronuclear DNA molecules that are adjacent in the micronucleus were also isolated and examined by DNA sequencing. The two macronuclear DNA molecules were found to be separated by only 90 base pairs in the micronuclear genome.
机译：在其生命周期的性阶段之后，间含量的氧化含量将其染色体微核的拷贝转化为含有短，线性DNA分子的通核核糖，其平均尺寸为2.2千碱基对。另外，在该过程中消除了微核基因组中的超过90％的DNA序列。我们研究了微核染色体中的Macronutal核DNA分子组织。发现Macronuclear DNA分子在微核DNA的两个克隆区段中聚集和分离小于550碱基对。还通过DNA测序分离和检查在微核中相邻的两个常规核DNA分子的重组克隆。发现两个常规核DNA分子在微核基因组中仅在90个碱基对中分离。

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