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首页> 美国卫生研究院文献>Molecular and Cellular Biology >Autogenous regulation of histone mRNA decay by histone proteins in a cell-free system.
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Autogenous regulation of histone mRNA decay by histone proteins in a cell-free system.

机译：在无细胞系统中组蛋白mRNA的自发调节由组蛋白降解。

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We tested the hypothesis that histone mRNA turnover is accelerated in the presence of free histone proteins. In an in vitro mRNA decay system, histone mRNA was degraded four- to sixfold faster in reaction mixtures containing core histones and a cytoplasmic S130 fraction than in reaction mixtures lacking these components. The decay rate did not change significantly when histones or S130 was added separately, suggesting either that the histones were modified and thereby activated by S130 or that additional factors besides histones were required. RecA, SSB (single-stranded binding), and histone proteins all formed complexes with histone mRNA, but only histones induced accelerated histone mRNA turnover. Therefore, the effect was not the result of random RNA-protein interactions. Moreover, histone proteins did not induce increased degradation of gamma globin mRNA, c-myc mRNA, or total poly(A)- or poly(A)+ polysomal mRNAs. This autoregulatory mechanism is consistent with the observed accumulation of cytoplasmic histone proteins in cells after DNA synthesis stops, and it can account, in part, for the rapid disappearance of histone mRNA at the end of S phase.
机译：我们测试了在游离组蛋白存在下组蛋白mRNA代谢加速的假设。在体外mRNA衰变系统中，与不含这些成分的反应混合物相比，含核心组蛋白和细胞质S130组分的反应混合物中组蛋白mRNA的降解速度快四到六倍。当单独添加组蛋白或S130时，衰减率没有明显变化，这表明组蛋白被S130修饰并因此被激活，或者除组蛋白外还需要其他因素。 RecA，SSB（单链结合）和组蛋白均与组蛋白mRNA形成复合物，但只有组蛋白诱导了组蛋白mRNA的加速代谢。因此，该效果不是随机RNA与蛋白质相互作用的结果。此外，组蛋白不会诱导γ球蛋白mRNA，c-myc mRNA或总poly（A）-或poly（A）+多体性mRNA的降解增加。这种自动调节机制与DNA合成停止后在细胞中观察到的细胞质组蛋白的积累是一致的，并且它可以部分解释S期末组蛋白mRNA的快速消失。

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