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首页> 外文期刊>Molecular and Cellular Biology >Autogenous regulation of histone mRNA decay by histone proteins in a cell-free system.
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Autogenous regulation of histone mRNA decay by histone proteins in a cell-free system.

机译：组蛋白蛋白在无细胞系统中的组蛋白mRNA衰减的自身调节。

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We tested the hypothesis that histone mRNA turnover is accelerated in the presence of free histone proteins. In an in vitro mRNA decay system, histone mRNA was degraded four- to sixfold faster in reaction mixtures containing core histones and a cytoplasmic S130 fraction than in reaction mixtures lacking these components. The decay rate did not change significantly when histones or S130 was added separately, suggesting either that the histones were modified and thereby activated by S130 or that additional factors besides histones were required. RecA, SSB (single-stranded binding), and histone proteins all formed complexes with histone mRNA, but only histones induced accelerated histone mRNA turnover. Therefore, the effect was not the result of random RNA-protein interactions. Moreover, histone proteins did not induce increased degradation of gamma globin mRNA, c-myc mRNA, or total poly(A)- or poly(A)+ polysomal mRNAs. This autoregulatory mechanism is consistent with the observed accumulation of cytoplasmic histone proteins in cells after DNA synthesis stops, and it can account, in part, for the rapid disappearance of histone mRNA at the end of S phase.
机译：我们测试了在自由组蛋白存在下加速组蛋白mRNA趋势的假设。在体外mRNA衰减系统中，组蛋白mRNA在含核心组蛋白的反应混合物中较快地降低四到六倍，并且比在缺乏这些组分的反应混合物中，细胞质S130级分。当分开添加组蛋白或S130时，衰减率没有显着变化，表明组蛋白被修饰，从而通过S130激活或者需要除了组蛋白之外的附加因子。 RECA，SSB（单链结合）和组蛋白蛋白质所有形成与组蛋白mRNA的复合物，但只有组蛋白诱导的加速组蛋白mRNA转换。因此，效果不是随机RNA蛋白质相互作用的结果。此外，组蛋白蛋白没有引起γ球蛋白mRNA，C-myc mRNA或全多种聚（a） - 或聚（a）+多晕MRNA的劣化。这种自动调节机制与DNA合成停止后观察到细胞组织组蛋白的聚细胞组蛋白蛋白的积累一致，并且它可以部分地考虑S期结束时组蛋白mRNA的快速消失。

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