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Maxicircle CR1 transcripts of Trypanosoma brucei are edited and developmentally regulated and encode a putative iron-sulfur protein homologous to an NADH dehydrogenase subunit.

机译：布鲁氏锥虫的Maxicircle CR1转录物经过编辑和发育调控并编码与NADH脱氢酶亚基同源的推定铁硫蛋白。

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The maxicircle of Trypanosoma brucei encodes components of the mitochondrial oxidative phosphorylation system, as do other mitochondrial DNAs, but maxicircle gene identification is complicated by extensive editing of some transcripts. We found that transcripts from the CR1 region were extensively edited, as are other transcripts from maxicircle regions which exhibit strong G versus C strand bias. Editing added 259 uridines and removed 46 uridines to produce an approximately 574-nucleotide mature mRNA. Partially edited cDNAs and potential guide RNAs were also characterized. Initiation and termination codons were created, and they defined an open reading frame encoding a predicted protein of 145 amino acids. This protein contains two iron-sulfur cysteine motifs and is homologous to a subunit of NADH dehydrogenase and to other electron-carrier proteins. Higher levels of both edited and unedited CR1 transcripts accumulated in bloodstream forms of the parasite than in procyclic forms, suggesting developmental regulation of CR1 gene expression.

机译：布氏锥虫的上颌环编码线粒体氧化磷酸化系统的组成部分，其他线粒体DNA一样，但是上颌环基因的鉴定由于对某些转录物的大量编辑而变得复杂。我们发现，CR1区域的转录本得到了广泛的编辑，maxicircle区域的其他转录本也表现出较强的G对C链偏向。编辑添加了259个尿苷，并去除了46个尿苷，以产生约574个核苷酸的成熟mRNA。还对部分编辑的cDNA和潜在的指导RNA进行了表征。创建了起始和终止密码子，它们定义了一个开放阅读框，该框编码了145个氨基酸的预测蛋白。该蛋白包含两个铁硫半胱氨酸基序，与NADH脱氢酶的一个亚基和其他电子载体蛋白同源。在寄生虫的血流形式中积累的编辑和未编辑的CR1转录物的水平都高于顺周期形式，这表明CR1基因表达的发育调控。

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期刊名称 Molecular and Cellular Biology
作者
A E Souza; P J Myler; K Stuart;
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年(卷),期 1992(12),5
年度 1992
页码 2100–2107
总页数 8
原文格式 PDF
正文语种
中图分类分子生物学;细胞生物学;
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1. Maxicircle CR1 transcripts of Trypanosoma brucei are edited and developmentally regulated and encode a putative iron-sulfur protein homologous to an NADH dehydrogenase subunit. [J] . A E Souza, P J Myler, K Stuart Molecular and Cellular Biology . 1992,第5期

机译：布鲁氏锥虫的Maxicircle CR1转录物被编辑和发展调节，并编码与NADH脱氢酶亚基同源的推定的铁硫蛋白。
2. Extensive editing of CR2 maxicircle transcripts of Trypanosoma brucei predicts a protein with homology to a subunit of NADH dehydrogenase. [J] . A E Souza, H H Shu, L K Read, Molecular and Cellular Biology . 1993,第11期

机译：布鲁氏锥虫CR2上环最大转录本的广泛编辑预测一种与NADH脱氢酶亚基同源的蛋白。
3. Guide RNAs for transcripts with developmentally regulated RNA editing are present in both life cycle stages of Trypanosoma brucei. [J] . D J Koslowsky, G R Riley, J E Feagin, Molecular and Cellular Biology . 1992,第5期

机译：布鲁氏锥虫的两个生命周期阶段均存在具有转录调控的RNA编辑的转录指导RNA。
4. Structural studies of RNA-editing proteins in Trypanosoma brucei. [D] . Wu, Mei-Ting. 2010

机译：布氏锥虫RNA编辑蛋白的结构研究。
5. Extensive editing of CR2 maxicircle transcripts of Trypanosoma brucei predicts a protein with homology to a subunit of NADH dehydrogenase. [O] . A E Souza, H H Shu, L K Read, 1993

机译：布鲁氏锥虫CR2上环最大转录本的广泛编辑预测一种与NADH脱氢酶亚基同源的蛋白。
6. Maxicircle CR1 transcripts of Trypanosoma brucei are edited and developmentally regulated and encode a putative iron-sulfur protein homologous to an NADH dehydrogenase subunit. [O] . Souza, A E, Myler, P J, Stuart, K 1992

机译：布鲁氏锥虫的Maxicircle CR1转录物经过编辑和发育调控，并编码与NADH脱氢酶亚基同源的推定铁硫蛋白。

Maxicircle CR1 transcripts of Trypanosoma brucei are edited and developmentally regulated and encode a putative iron-sulfur protein homologous to an NADH dehydrogenase subunit.

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