• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Molecular and Cellular Biology >Stem-Loop Binding Protein the Protein That Binds the 3′ End of Histone mRNA Is Cell Cycle Regulated by Both Translational and Posttranslational Mechanisms
【2h】

Stem-Loop Binding Protein the Protein That Binds the 3′ End of Histone mRNA Is Cell Cycle Regulated by Both Translational and Posttranslational Mechanisms

机译：茎环结合蛋白是结合组蛋白mRNA 3末端的蛋白其细胞周期受翻译和翻译后机制的调节

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The expression of the replication-dependent histone mRNAs is tightly regulated during the cell cycle. As cells progress from G1 to S phase, histone mRNA levels increase 35-fold, and they decrease again during G2 phase. Replication-dependent histone mRNAs are the only metazoan mRNAs that lack polyadenylated tails, ending instead in a conserved stem-loop. Much of the cell cycle regulation is posttranscriptional and is mediated by the 3′ stem-loop. A 31-kDa stem-loop binding protein (SLBP) binds the 3′ end of histone mRNA. The SLBP is necessary for pre-mRNA processing and accompanies the histone mRNA to the cytoplasm, where it is a component of the histone messenger RNP. We used synchronous CHO cells selected by mitotic shakeoff and HeLa cells synchronized at the G1/S or the M/G1 boundary to study the regulation of SLBP during the cell cycle. In each system the amount of SLBP is regulated during the cell cycle, increasing 10- to 20-fold in the late G1 and then decreasing in the S/G2 border. SLBP mRNA levels are constant during the cell cycle. SLBP is regulated at the level of translation as cells progress from G1 to S phase, and the protein is rapidly degraded as they progress into G2. Regulation of SLBP may account for the posttranscriptional component of the cell cycle regulation of histone mRNA.
机译：复制依赖性组蛋白mRNA的表达在细胞周期中受到严格调节。随着细胞从G1期发展到S期，组蛋白mRNA水平增加35倍，而在G2期又降低。复制依赖的组蛋白mRNA是仅有的缺少聚腺苷酸化尾巴的后生mRNA，而是以保守的茎环结尾。许多细胞周期调节是转录后的，并由3'茎环介导。 31 kDa茎环结合蛋白（SLBP）与组蛋白mRNA的3'末端结合。 SLBP对于mRNA预处理是必需的，并且是组蛋白mRNA与细胞质的结合体，是组蛋白信使RNP的组成部分。我们使用通过有丝分裂摇晃选择的同步CHO细胞和在G1 / S或M / G1边界同步的HeLa细胞来研究SLBP在细胞周期中的调控。在每个系统中，SLBP的量在细胞周期中受到调节，在晚期G1中增加10到20倍，然后在S / G2边界中减少。 SLBP mRNA水平在细胞周期中是恒定的。随着细胞从G1进入S期，SLBP在翻译水平受到调控，并且蛋白质在进入G2时迅速降解。 SLBP的调节可能解释了组蛋白mRNA细胞周期调节的转录后成分。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号