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首页> 美国卫生研究院文献>Molecular and Cellular Biology >Study of the Functional Interaction between Mcp Insulators from the Drosophila bithorax Complex: Effects of Insulator Pairing on Enhancer-Promoter Communication
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Study of the Functional Interaction between Mcp Insulators from the Drosophila bithorax Complex: Effects of Insulator Pairing on Enhancer-Promoter Communication

机译：果蝇bithorax复合体的Mcp绝缘子之间的功能相互作用的研究：绝缘子配对对增强子-启动子通讯的影响

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Boundary elements have been found in the Abd-B 3′ cis-regulatory region, which is subdivided into a series of iab domains. Previously, a 340-bp insulator-like element, M340, was identified in one such 755-bp Mcp fragment linked to the PcG-dependent silencer. In this study, we identified a 210-bp core that was sufficient for pairing of sequence-remote Mcp elements. In two-gene transgenic constructs with two Mcp insulators (or their cores) surrounding yellow, the upstream yeast GAL4 sites were able to activate the distal white only if the insulators were in the opposite orientations (head-to-head or tail-to-tail), which is consistent with the looping/bypass model. The same was true for the efficiency of the cognate eye enhancer, while yellow thus isolated in the loop from its enhancers was blocked more strongly. These results indicate that the relative placement and orientation of insulator-like elements can determine proper enhancer-promoter communication.
机译：在Abd-B 3'顺式调控区域中发现了边界元素，该区域可细分为一系列iab结构域。以前，在一个与PcG依赖的沉默子连锁的755 bp Mcp片段中发现了一个340 bp的类似绝缘子的分子M 340 。在这项研究中，我们确定了一个210 bp的核心，足以与序列远程Mcp元件配对。在带有两个环绕黄色的两个Mcp绝缘子（或它们的核心）的双基因转基因构建体中，只有当绝缘子的方向相反（头对头或尾对头）时，上游酵母GAL4位点才能激活远端白色。尾部），与循环/旁路模型一致。同源眼增强剂的功效也是如此，而从环中与其增强剂隔离的黄色被更强烈地阻断。这些结果表明，类似绝缘子的元件的相对位置和方向可以确定适当的增强子-促进子连通。

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