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Adaptation by stochastic switching of a monostable genetic circuit in Escherichia coli

机译：通过随机切换单稳态遗传电路来适应大肠杆菌

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摘要

Stochastic switching is considered as a cost-saving strategy for adaptation to environmental challenges. We show here that stochastic switching of a monostable circuit can mediate the adaptation of the engineered OSU12-hisC Escherichia coli strain to histidine starvation. In this strain, the hisC gene was deleted from the His operon and placed under the control of a monostable foreign promoter. In response to histidine depletion, the OSU12-hisC population shifted to a higher HisC expression level, which is beneficial under starving conditions but is not favoured by the monostable circuit. The population shift was accompanied by growth recovery and was reversible upon histidine addition. A weak directionality in stochastic switching of hisC was observed in growing microcolonies under histidine-free conditions. Directionality and fate decision were in part dependent on the initial cellular status. Finally, microarray analysis indicated that OSU12-hisC reorganized its transcriptome to reach the appropriate physiological state upon starvation. These findings suggest that bacteria do not necessarily need to evolve signalling mechanisms to control gene expression appropriately, even for essential genes.
机译：随机切换被认为是适应环境挑战的一种节省成本的策略。我们在这里显示，单稳态电路的随机切换可以介导工程OSU12-hisC大肠杆菌菌株对组氨酸饥饿的适应。在该菌株中，hisC基因从His操纵子中缺失，并置于单稳态外源启动子的控制下。响应组氨酸消耗，OSU12-hisC群体转移到更高的HisC表达水平，这在饥饿条件下是有益的，但不受单稳态电路的支持。群体转移伴随着生长恢复，并且在加入组氨酸后是可逆的。在无组氨酸条件下生长的小菌落中观察到hisC随机转换的弱方向性。方向性和命运决定部分取决于初始细胞状态。最后，微阵列分析表明OSU12-hisC在饥饿时重组其转录组以达到适当的生理状态。这些发现表明，即使对于必需基因，细菌也不一定需要进化信号机制来适当地控制基因表达。

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