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Determination of Essential Oil Composition from Osmanthusfragrans Tea by GC-MS Combined with a Chemometric Resolution Method

机译:桂花挥发油成分的测定GC-MS结合化学计量学解析方法测定的苦茶

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摘要

The essential oils of the Osmanthus fragrans tea (OFT), Osmanthus fragrans (OF) and green tea (GT) had been extracted by steam distillation and analyzed by gas chromatography-mass spectrometry (GC-MS) with the help of heuristic evolving latent projections (HELP), an effective chemometric resolution method (CRM). The overlapping peak clusters were resolved into pure chromatograms and pure mass spectra with HELP. The qualitative analysis was performed by similarity searches in the National Institute of Standards and Technology (NIST) mass spectra database with the obtained pure mass spectrum of each component. Identification of some compounds was also assisted by comparison of temperature-programmed retention indices (PTRI) with authentic standards included in our own laboratory database under construction. The quantitative results were obtained by overall volume integration (OVI) method. A total of 67, 73 and 53 components in essential oils of the OFT, OF and GT were identified, accounting for 90.83%, 93.65% and 89.97% total contents of the essential oil of OFT, OF and GT, respectively.
机译:通过蒸汽蒸馏提取桂花茶(OFT),桂花(OF)和绿茶(GT)的香精油,并借助启发式进化潜伏投影法通过气相色谱-质谱法(GC-MS)进行分析。 (HELP),一种有效的化学计量拆分方法(CRM)。使用HELP将重叠的峰簇解析为纯色谱图和纯质谱。通过在美国国家标准技术研究院(NIST)质谱数据库中的相似性搜索,对获得的每种组分的纯质谱进行了定性分析。通过比较程序升温保留指数(PTRI)与我们自己正在建设中的实验室数据库中包含的真实标准,还有助于鉴定某些化合物。定量结果是通过整体体积积分(OVI)方法获得的。在OFT,OF和GT的香精油中共鉴定出67、73和53种成分,分别占OFT,OF和GT香精油总含量的90.83%,93.65%和89.97%。

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