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首页> 美国卫生研究院文献>Molecules >Volatile Fingerprint of Italian Populations of Orchids Using Solid Phase Microextraction and Gas Chromatography Coupled with Mass Spectrometry
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Volatile Fingerprint of Italian Populations of Orchids Using Solid Phase Microextraction and Gas Chromatography Coupled with Mass Spectrometry

机译：固相微萃取和气相色谱-质谱联用分析意大利兰花种群的挥发性指纹图谱

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摘要

The volatile fraction of Ophrys sphegodes Mill. subsp. sphegodes, Ophrys bertolonii subsp. benacensis (Reisigl) O. Danesch, E. Danasch & Ehrend. and Neotinea tridentata (Scop.) R.M. Bateman, Pridgeon & M.W. Case, three orchid species with different pollinator attraction strategies, sampled in vivo and in situ, were evaluated by headspace solid phase microextraction coupled with gas-chromatography and mass spectrometry. The results were compared with the volatile compounds emitted by flowering plant samples picked from the same populations of orchid species. Hydrocarbons, aldehydes, alcohols and terpenes were the major constituents of “in vivo” orchid scents and some distinctive differences in volatile metabolite composition were observed between Ophrys and Neotinea species. Moreover, the odour bouquets of the picked flowering plant samples were different from the in vivo ones and in particular different proportions of the various terpenes and an increase of α-pinene were observed. In conclusion HS/SPME GCMS proved to be a suitable technique for analyzing and distinguishing the volatile fingerprint of different orchid species, sampled in vivo and in situ in a non-disruptive way, with potentially great advantages for ecophysiological studies of rare and endangered species.
机译：Ophrys sphegodes Mill的挥发性部分。亚种sphegodes，Ophrys bertolonii亚种。本纳克斯（Reisigl）O. Danesch，E.Danasch和Ehrend。和Neotinea tridentata（Scop。）R.M。 Bateman，Pridgeon和M.W. Case用顶空固相微萃取结合气相色谱和质谱法评估了在体内和原位采样的三种具有不同授粉媒介吸引策略的兰花物种。将结果与从相同兰花物种种群中采集的开花植物样品释放的挥发性化合物进行了比较。碳氢化合物，醛，醇和萜烯是“体内”兰花气味的主要成分，并且在Ophrys和Neotinea物种之间观察到挥发性代谢物成分的一些明显差异。此外，采摘的开花植物样品的气味花束与体内的气味花束不同，尤其是各种萜烯的比例不同并且观察到α-increase烯的增加。总而言之，HS / SPME GCMS被证明是一种用于分析和区分不同兰花物种的挥发性指纹的合适技术，以无干扰的方式在体内和原位采样，对于稀有和濒危物种的生态生理研究具有潜在的巨大优势。

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