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Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica

机译:带有蒸发光散射检测器的HPLC系统中馏分收集的可行性:大型果胶的分析

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摘要

The use of a liquid chromatography (LC) splitter inserted between an HPLC column and an evaporative light scattering detector (ELSD) is described. This paper aims to show the feasibility of using the splitter in an HPLC-ELSD system to fractionate a model mixture of analytes, namely salicin (2-(hydroxymethyl)-phenyl-β-d-glucopyranoside) and glucose. The retention factors and efficiency of the separation were studied under various temperatures and water contents in the mobile phase in order to clarify the mechanism of polyols separation on a diol column under the conditions of hydrophilic liquid chromatography (HILIC). Finally, the system was applied to a biological sample (a lyophilized colony gel of Pectinatella magnifica), where the presence of fructose and glucose was confirmed.
机译:描述了在液相色谱柱和蒸发光散射检测器(ELSD)之间插入的液相色谱(LC)分离器的使用。本文旨在展示在HPLC-ELSD系统中使用分离器分离水杨素(2-(羟甲基)-苯基-β-d-吡喃葡萄糖苷)和葡萄糖的模型混合物的可行性。研究了在各种温度和流动相中的水含量下分离的保留因子和分离效率,以阐明在亲水液相色谱(HILIC)条件下在二醇柱上进行多元醇分离的机理。最后,将该系统应用于生物样品(巨大果胶冻干菌落凝胶),其中证实了果糖和葡萄糖的存在。

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