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High-Performance Liquid Chromatography with Diode Array Detector and Electrospray Ionization Ion Trap Time-of-Flight Tandem Mass Spectrometry to Evaluate Ginseng Roots and Rhizomes from Different Regions

机译:高效液相色谱带二极管阵列检测器和电喷雾电离离子阱飞行时间串联质谱用于评估不同地区的人参根和根茎

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摘要

Ginseng, Panax ginseng C. A. Meyer, is an industrial crop in China and Korea. The functional components in ginseng roots and rhizomes are characteristic ginsenosides. This work developed a new high-performance liquid chromatography coupled with electrospray ionization ion trap time-of-flight multistage mass spectrometry (LC–ESI-IT-TOF-MSn) method to identify the triterpenoids. Sixty compounds (>1–>60) including 58 triterpenoids were identified from the ginseng cultivated in China. Substances >1, >2, >7, >15–>20, >35, >39, >45–>47, >49, >55–>57, >59, and >60 were identified for the first time. To evaluate the quality of ginseng cultivated in Northeast China, this paper developed a practical liquid chromatography–diode array detection (LC–DAD) method to simultaneously quantify 14 interesting ginsenosides in ginseng collected from 66 different producing areas for the first time. The results showed the quality of ginseng roots and rhizomes from different sources was different due to growing environment, cultivation technology, and so on. The developed LC–ESI-IT-TOF-MSn method can be used to identify many more ginsenosides and the LC–DAD method can be used not only to assess the quality of ginseng, but also to optimize the cultivation conditions for the production of ginsenosides.
机译:人参,人参C. A. Meyer,是中国和韩国的工业作物。人参根和根茎中的功能成分是特征性人参皂甙。这项工作开发了一种新型的高效液相色谱仪,结合电喷雾电离离子阱飞行时间多级质谱分析法(LC-ESI-IT-TOF-MS n )来鉴定三萜类化合物。从中国种植的人参中鉴定出60种化合物(> 1 – > 60 ),其中包括58种三萜。物质> 1 ,> 2 ,> 7 ,> 15 – > 20 ,> 35 ,> 39 ,> 45 – > 47 ,> 49 ,> 55 首次识别出> 57 ,> 59 和> 60 。为了评估中国东北种植的人参的质量,本文开发了一种实用的液相色谱-二极管阵列检测(LC-DAD)方法,以同时对来自66个不同产地的人参中的14种有趣的人参皂苷进行首次定量。结果表明,不同的人参根茎和根茎的质量因生长环境,栽培技术等的不同而不同。发达的LC-ESI-IT-TOF-MS n 方法可用于鉴定更多的人参皂苷,而LC-DAD方法不仅可用于评估人参质量,而且还可用于优化人参皂甙生产的培养条件。

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