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首页> 外文学位 >Analytical characterization of anthocyanins from natural products by reverse-phase liquid chromatography-photodiode array-electrospray ionization-ion trap-time of flight mass spectrometry.
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Analytical characterization of anthocyanins from natural products by reverse-phase liquid chromatography-photodiode array-electrospray ionization-ion trap-time of flight mass spectrometry.

机译：反相液相色谱-光电二极管阵列-电喷雾电离-离子阱-飞行时间质谱对天然产物中的花色苷进行分析表征。

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摘要

Anthocyanins are known to be one of the most powerful phytochemical antioxidant and believed to have a positive influence on a variety of health conditions. Numerous studies continue on these compounds that are readily found in most plants. Red hybrid-tea rose petals and wild blueberries were used as model systems for optimizing the extraction and identification process of anthocyanins in plant material using high performance liquid chromatography (HPLC) and mass spectrometry (MS) without the use of authentic standards. A number of extraction parameters, including sample homogenization, solvent selection and acid type and amount, were varied to examine the effects on anthocyanin extraction from blueberries. Individual anthocyanins within the extract solution were separated by reverse phase HPLC and identified by elution order and tandem mass spectrometry. Further definitive identification of anthocyanins from other isobaric flavonoid species was demonstrated by multi-dimensional tandem mass spectrometry fragmentation (MSn). Cyanidin-3,5-diglucoside and pelargonidin-3,5-diglucoside were isolated from an extract of freeze dried rose petals via fractionation. These compounds were then structurally-characterized by multi-stage mass spectrometry fragmentation (up to MS 6). Variations in the collision energy of the ion trap resulted in cleavage of the glycosides at the MS/MS, and MS3 stages; fragmentation of the anthocyanidin was recorded at the MS4 stage and beyond. Computational modeling was used to investigate the cross-ring cleavage fragment ions of cyanidin.
机译：花青素是最强大的植物化学抗氧化剂之一，据信对多种健康状况都有积极影响。继续对大多数植物中容易找到的这些化合物进行大量研究。红色杂种茶玫瑰花瓣和野生蓝莓用作模型系统，可使用高效液相色谱（HPLC）和质谱（MS）优化植物材料中花色苷的提取和鉴定过程，而无需使用真实的标准。改变了许多提取参数，包括样品的均质性，溶剂选择以及酸的种类和数量，以研究其对蓝莓花色苷提取的影响。通过反相HPLC分离提取液中的各个花色苷，并通过洗脱顺序和串联质谱进行鉴定。多维串联质谱碎裂（MSn）证明了其他同量异黄酮类化合物中花色苷的进一步确定。通过分级分离，从冷冻干燥的玫瑰花瓣的提取物中分离出Cyanidin-3,5-二葡萄糖苷和pelargonidin-3,5-二葡萄糖苷。然后通过多级质谱裂解（至MS 6）对这些化合物进行结构表征。离子阱碰撞能量的变化导致在MS / MS和MS3阶段糖苷的裂解。在MS4阶段及以后记录了花青素的断裂。计算模型被用来研究花青素的跨环裂解碎片离子。

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  • 作者

    Barnes, Jeremy S.;

  • 作者单位

    The University of Texas at Arlington.;

  • 授予单位 The University of Texas at Arlington.;
  • 学科 Chemistry Analytical.;Chemistry Agricultural.
  • 学位 M.S.
  • 年度 2010
  • 页码 97 p.
  • 总页数 97
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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