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首页> 美国卫生研究院文献>Molecules >Design Synthesis and Use of Peptides Derived from Human Papillomavirus L1 Protein for the Modification of Gold Electrode Surfaces by Self-Assembled Monolayers
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Design Synthesis and Use of Peptides Derived from Human Papillomavirus L1 Protein for the Modification of Gold Electrode Surfaces by Self-Assembled Monolayers

机译：人乳头瘤病毒L1蛋白衍生肽的设计合成及其应用用于通过自组装单层膜修饰金电极表面

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In order to obtain gold electrode surfaces modified with Human Papillomavirus L1 protein (HPV L1)-derived peptides, two sequences, SPINNTKPHEAR and YIK, were chosen. Both have been recognized by means of sera from patients infected with HPV. The molecules, Fc-Ahx-SPINNTKPHEAR, Ac–C–Ahx-(Fc)KSPINNTKPHEAR, Ac–C–Ahx-SPINNTKPHEAR(Fc)K, C–Ahx–SPINNTKPHEAR, and (YIK)2–Ahx–C, were designed, synthesized, and characterized. Our results suggest that peptides derived from the SPINNTKPHEAR sequence, containing ferrocene and cysteine residues, are not stable and not adequate for electrode surface modification. The surface of polycrystalline gold electrodes was modified with the peptides C-Ahx-SPINNTKPHEAR or (YIK)2-Ahx-C through self-assembly. The modified polycrystalline gold electrodes were characterized via infrared spectroscopy and electrochemical measurements. The thermodynamic parameters, surface coverage factor, and medium pH effect were determined for these surfaces. The results indicate that surface modification depends on the peptide sequence (length, amino acid composition, polyvalence, etc.). The influence of antipeptide antibodies on the voltammetric response of the modified electrode was evaluated by comparing results obtained with pre-immune and post-immune serum samples.
机译：为了获得用人乳头瘤病毒L1蛋白（HPV L1）衍生的肽修饰的金电极表面，选择了两个序列SPINNTKPHEAR和YIK。两者均已被HPV感染患者的血清所识别。设计了分子Fc-Ahx-SPINNTKPHEAR，Ac–C–Ahx-（Fc）KSPINNTKPHEAR，Ac–C–Ahx-SPINNTKPHEAR（Fc）K，C–Ahx–SPINNTKPHEAR和（YIK）2-Ahx–C ，合成和表征。我们的结果表明，衍生自SPINNTKPHEAR序列的含有二茂铁和半胱氨酸残基的肽不稳定且不足以进行电极表面修饰。通过自组装，用肽C-Ahx-SPINNTKPHEAR或（YIK）2-Ahx-C修饰多晶金电极的表面。修饰的多晶金电极通过红外光谱和电化学测量来表征。确定了这些表面的热力学参数，表面覆盖系数和中等pH值。结果表明表面修饰取决于肽序列（长度，氨基酸组成，多价等）。通过比较免疫前和免疫后血清样品获得的结果，评估抗肽抗体对修饰电极的伏安响应的影响。

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